Manipulation of tRNA to Enhance Biologic Production

操纵 tRNA 以增强生物制品生产

• 批准号: BB/S018727/1
• 负责人: Robert White
• 金额: $ 24.84万
• 依托单位: University of York
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS018727%2F1
• 关键词: Manipulation; tRNA; Enhance; Biologic; Production

Biologic production is a multibillion dollar industry, providing substantial healthcare benefits and unlimited opportunity for expansion. Funded by a BBSRC Industrial Biotechnology Catalyst Early Stage Translation Award, my lab developed a novel synthetic biology strategy that boosts product quality and yield in proof-of-principle experiments. Follow-on funding is sought to support conclusive tests of our strategy using proprietary platforms and biologics under conditions of industrial production, in collaborations with multinational companies. Our strategy is based on measuring and manipulating tRNAs required for biologic synthesis. Some tRNAs are much more abundant than others and scarcity of a single tRNA can restrict production. However, relative levels of tRNAs have not been measured in industrial contexts. We propose to fill this key knowledge gap and remediate deficiencies by tRNA supplementation and improved codon optimization.

生物生产是一个数十亿美元的行业，为扩张提供了可观的医​​疗保健和无限的机会。由BBSRC工业生物技术催化剂早期翻译奖资助，我的实验室制定了一种新型的合成生物学策略，可提高原则实验证明的产品质量和产量。寻求后续资金，以与跨国公司合作，在工业生产条件下使用专有平台和生物制剂来支持我们战略的结论性测试。我们的策略是基于测量和操纵生物合成所需的tRNA。某些trna比其他trna更丰富，单个tRNA的稀缺性可以限制产生。但是，在工业环境中尚未测量TRNA的相对水平。我们建议通过补充tRNA并改善密码子优化来填补这一关键知识差距，并补充缺陷。