STUDY OF INTRAOCULAR TRANSPORT

眼内运输的研究

• 批准号: 3255404
• 负责人: VENKAT N REDDY
• 金额: $ 26.45万
• 依托单位: OAKLAND UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-05-01 至 1989-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3255404
• 关键词: active transport; aging; aminoacid transport; ascorbate; blood aqueous barrier; cataract; cell aggregation; congenital vision disorder; detoxification; gel electrophoresis; glaucoma; glutathione; histochemistry /cytochemistry; human tissue; hydrogen peroxide; intraocular fluid; lens; lens proteins; membrane permeability; radioassay; radiobiology; retinal pigment epithelium; uvea ciliary body

The long term objectives are to elucidate the mechanisms of transport of various substances into and out of the lens and across the blood-aqueous and blood-vitreous barriers and to assess how the abberations in these mechanisms contribute to the pathogenesis of cataract and other ocular abnormalities. The multifaceted approach to this broad program involves studies of the metabolism and function of glutathione in lens and other ocular structures in relation to membrane permeability, active transport, oxidative damage, and lens protein aggregation. The origin of H202 in aqueous humor of various species, its role in cataract formation and the mechanism of its detoxification by the lens will be examined. The role of glycoproteins and other membrane components in lens permeability will be investigated in genetic cataracts in Philly mouse and in cataracts induced by X-ray, tryptophan- and selenium-deficiencies as well as in human lens. Other aspects of these studies are concerned with the effect of cations on protein synthesis in X-ray cataract, and the multiple effect of low levels of X-ray and other cataractogenic insults in experimental animals. Investigations of transport characteristics of amino acids across blood-aqueous and blood-vitreous barrier will be continued and extended; these approaches utilize in vivo transport into ocular chambers in rabbits as well as isolated ciliary body in vitro and arterially perfused enucleated eyes. The study to establish a number of biochemical parameters, such as free amino acids, glutathione, cations and the enzymes of glutathione metabolism and hexosemonophosphate shunt in human lens will be continued. In addition to providing new knowledge on the biochemistry of the human lens correlation between the changes in these parameters and the nature and degree of opacity in human senile cataracts, classified according to Cooperative Cataract Research Group criteria, will be sought through the use of the Prophet computer system. While the proposed studies on the lens bear directly on the etiology of experimental and human senile cataracts, those concerned with blood-aqueous barrier are relevant to intraocular fluid dynamics and thus to glaucoma.

长期目标是阐明运输机制 各种物质进入镜头和流入血液 和血液障碍，并评估这些差异如何 机制有助于白内障和其他眼的发病机理 异常。 该广泛计划的多方面方法涉及 研究晶状体和其他谷胱甘肽的代谢和功能 与膜渗透性，主动转运相关的眼部结构 氧化损伤和晶状体蛋白聚集。 H202的起源 各种物种的水性幽默，其在白内障形成中的作用和 将检查其透镜排毒的机制。 的作用 透镜渗透性中的糖蛋白和其他膜成分将是 在费城小鼠和白内障中的遗传性白内障研究 由X射线，色氨酸和硒缺陷以及人类镜头中的X射线。 这些研究的其他方面涉及阳离子对 X射线白内障中的蛋白质合成，低水平的多重作用 实验动物中的X射线和其他白内障侮辱。 研究跨氨基酸的运输特征 血液和血液屏障将继续并延伸； 这些方法利用体内运输到兔子中的眼室 以及孤立的睫状体体外和动脉灌注 浓眼睛的眼睛。 这项研究建立了许多生化 参数，例如游离氨基酸，谷胱甘肽，阳离子和酶 谷胱甘肽的代谢和人镜中的六磷酸分流的 继续。 除了提供有关生物化学的新知识 这些参数变化与 人类老年白内障的性质和不透明度，分类 根据合作性白内障研究小组标准，将寻求 通过使用先知计算机系统。 而拟议的研究 镜头上直接对实验和人类老年病因 白内障，与血液屏障有关的人与 眼内流体动力学，因此是青光眼。