DEVELOPMENT AND REORGANIZATION OF PRENATAL VISUAL SYSTEM

产前视觉系统的发育和重组

• 批准号: 3258471
• 负责人: LEO M CHALUPA
• 金额: $ 13.26万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-08-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3258471
• 关键词: axon; cats; developmental neurobiology; evolution; fluorescence; lateral geniculate body; neural plasticity; neuroanatomy; neurophysiology; retinal ganglion; single cell analysis; visual fields; visual pathways

Three sets of experiments are proposed to continue our research on the prenatal development of the mammalian visual system. In the next 5 years we will focus on the normal ontogeny of retinal ganglion cells and their axonal projection patterns. First, we will study the ontogeny of regional specialization within the ganglion cell layer. In these experiments retinal ganglion cells will be identified unequivocally by labeling these neurons following injections of the retrograde tracer horseradish peroxidase into the optic chiasm of fetal animals at known gestational ages. As a result it will be possible to study the spatial and temporal patterns of retinal ganglion cell outgrowth as well as the morphological differentiation of these neurons. This information is essential in order to increase our understanding of the processes underlying the maturation of the retinal ganglion cell layer. In the second set of experiments, the formation of retinal nasotemporal decussation patterns will be studied by injecting different fluorescent tracers (diamidino yellow and rhodamine-labeled microspheres) into the main retinorecipient regions on each side of the brain. This will permit an accurate assessment of the degree of nasotemporal overlap in the distribution of ganglion cells whose axons project to the same or opposite side of the brain. It will also be feasible to determine the presence of bilaterally projecting ganglion cells. In the third set of studies, the topographical organization of developing crossed and uncrossed retinofugal projections will be examined using two complementary anatomical methods. These studies will permit us to assess the degree to which developmental refinements and corrections may contribute to the topographical organization of retinal projections. The immediate significance of this research program is to increase our understanding of the events underlying the normal development of the visual system. These results will also provide new knowledge of general principles governing the development of the mammalian brain.

提出了三套实验，以继续我们的研究 哺乳动物视觉系统的产前发展。 在接下来的5年中 我们将重点放在视网膜神经节细胞及其其的正常本身上 轴突投影模式。 首先，我们将研究区域的个体发育 神经节细胞层中的专业化。 在这些实验中 视网膜神经节细胞将通过标记这些明确鉴定 在注射逆行示踪者辣根后的神经元 过氧化物酶进入已知妊娠胎儿动物的视神经chiasm 年龄。 结果，可以研究空间和时间 视网膜神经节细胞出生的模式以及形态学 这些神经元的区分。 此信息至关重要 增加我们对成熟过程基础的过程的理解 视网膜神经节细胞层。 在第二组实验中， 视网膜鼻腔延迟模式的形成将由 注入不同的荧光示踪剂（二氨基黄色和 若丹明标记的微球）进入主视网膜公位区域 大脑的每一侧。 这将允许对 神经节细胞分布中的鼻颞重叠程度 轴突将大脑的相同或对面的侧面投影。 也将是 可行以确定双侧投射神经节的存在 细胞。 在第三组研究中， 将检查开发交叉和未划线的视网膜预测 使用两种互补的解剖方法。 这些研究将使我们 评估发展性和更正的程度 为视网膜预测的地形组织做出贡献。 这 该研究计划的直接意义是增加我们的 理解视觉正常发展的事件 系统。 这些结果还将提供一般知识 管理哺乳动物大脑发展的原则。