WOUND HEALING ACCELERATION USING NATURAL PRODUCTS

使用天然产品加速伤口愈合

基本信息

批准号：
2810705
负责人：
GERALD B HAMMOND
金额：
$ 10.75万
依托单位：
UNIVERSITY OF MASSACHUSETTS DARTMOUTH
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-05-01 至 2003-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2810705
关键词：
DNA topoisomerases Peru antineoplastics bioassay cell migration cell proliferation chemical structure function drug discovery /isolation enzyme inhibitors medicinal plants plant extracts wound healing

项目摘要

This renewal proposal describes an interdisciplinary, systematic and targeted study of Peruvian plants with cicatrizant (wound-healing) activity. Although cicatrizant plants constitute the main focus of this proposal, extracts will also be screened for potential antineoplasic activity. The diverse topography, semitropical climate and unique cultural heritage of Peru contribute to making this country a desirable place for bioprospecting. Cicatrizant agents could help patients with illness such as diabetes, gastric ulcers or varicose ulcers, whose wounds do not scar easily. Notwithstanding these medical benefits, the scientific study of wound-healing plants may help uncover whether a connection can be established between injuries, healing and the treatment of proliferative disorders. Recent work has uncovered evidence that strengthens a correlation between injury and carcinogenesis. The study of cicatrizant plants and the mechanisms by which their active principle(s) operate, will reveal natural agents capable of promoting tissue regeneration. This investigation will shed light on externally promoted healing mechanisms triggered by acute and, perhaps chronic, injury. A five-step approach to wound-healing natural products has been designed: 1. Characterization of the active principle from Anredera diffusa and bioassay-guided fractionation of Jatropa curcas latex. The former was isolated during the previous grant period. 2. Structure-activity relationship studies on taspine and (+)-anymol, the two cicatrizant principles found in Croton lechleri and Peperomia galioides, respectively. The latter was isolated and identified during the previous grant period. 3. Cell migration and cell proliferation studies to probe mechanism of action for cicatrizant principles found in Peperomia galioides, Anredera diffusa and Jatropa curcas. 4. Characterization of the active principle from an extract who failed to show in vivo wound-healing activity during the previous grant period but tested positive in our anticancer screening assay. This corresponds to a potential topoisomerase II inhibitor from Campsiandra angustifolia. 5. Scientific evaluation of crude extracts from newly identified Peruvian plants with cicatrizant and anticancer activity. The long-term objective of this research is to strengthen the international and multidisciplinary collaboration between scientists from Peru and the United States, with the aim of linking conservation of biological diversity with sustained economic management of medicinal plants needed for the discovery and development of pharmaceuticals to improve human health.

该更新提案描述了对秘鲁植物具有愈合（伤口愈合）活性的跨学科、系统和有针对性的研究。虽然疤痕植物构成了该提案的主要重点是，还将筛选提取物的潜在抗肿瘤活性。秘鲁多样的地形、亚热带气候和独特的文化遗产为使该国成为生物勘探的理想之地做出贡献。疤痕剂可以帮助患有糖尿病、胃溃疡或静脉曲张性溃疡等伤口不易结疤的患者。尽管有这些医疗福利，对伤口愈合植物的科学研究可能有助于揭示损伤、愈合和增殖性疾病的治疗之间是否存在联系。最近的工作发现了加强损伤和致癌之间相关性的证据。对疤痕植物及其有效成分发挥作用的机制的研究将揭示能够促进组织再生的天然物质。这项研究将揭示由急性或慢性损伤引发的外部促进的愈合机制。伤口愈合天然产品的五步法设计： 1. 白花蛇舌草活性成分的表征和麻疯树乳胶的生物测定引导分馏。前者在上一次资助期间被隔离时期。 2. 分别在巴豆和胡椒中发现的两种愈合原理taspine和(+)-anymol的构效关系研究。后者是在上一个资助期间被隔离和识别。 3. 细胞迁移和细胞增殖研究，探讨胡椒草中发现的愈合原理的作用机制 galioides、Anredera diffusa 和 Jatropa curcas。 4. 提取物中活性成分的表征，该提取物在之前的研究中未能表现出体内伤口愈合活性授予期但在我们的抗癌筛查试验中测试呈阳性。这对应于来自 Campsiandra angustifolia 的潜在拓扑异构酶 II 抑制剂。 5. 对新鉴定的秘鲁植物粗提物的结疤和抗癌活性进行科学评价。这项研究的长期目标是加强秘鲁和美国科学家之间的国际和多学科合作，旨在将生物多样性保护与发现和开发药物以改善人类健康所需的药用植物的持续经济管理联系起来。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

In vivo wound-healing activity of oleanolic acid derived from the acid hydrolysis of Anredera diffusa.

来自白花蛇舌草酸水解的齐墩果酸的体内伤口愈合活性。

DOI：
发表时间：
2006-06
期刊：
影响因子：
0
作者：
Moura;Villegas, Leon F;Marcalo, Ana;Vaisberg, Abraham J;Hammond, Gerald B
通讯作者：
Hammond, Gerald B

Use of a modified microplate bioassay method to investigate antibacterial activity in the Peruvian medicinal plant Peperomia galioides.

DOI：
10.1016/j.jep.2004.06.013
发表时间：
2004-10-01
期刊：
Journal of ethnopharmacology
影响因子：
5.4
作者：
Richard D Langfield;F. Scarano;Mary E Heitzman;Miwako Kondo;G. Hammond;C. Neto
通讯作者：
C. Neto

Antibacterial activity of some Peruvian medicinal plants from the Callejon de Huaylas.

Callejon de Huaylas 的一些秘鲁药用植物的抗菌活性。

DOI：
发表时间：
2002-01
期刊：
影响因子：
5.4
作者：
Neto, Catherine C;Owens, Charles W;Langfield, Richard D;Comeau, Anthony B;Onge, Julie St;Vaisberg, Abraham J;Hammond, Gerald B
通讯作者：
Hammond, Gerald B

Ethnobotany, phytochemistry and pharmacology of Uncaria (Rubiaceae).

钩藤（茜草科）的民族植物学、植物化学和药理学。