DOES EXPOSURE OF HUMAN CELLS TO EM FIELDS CAUSE CANCER

人体细胞暴露于电磁场会导致癌症吗

• 批准号: 3254041
• 负责人: Ann S Henderson
• 金额: $ 46.56万
• 依托单位: HUNTER COLLEGE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-21 至 1996-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3254041
• 关键词: DNA replication; biological signal transduction; cell line; electrofocusing; electromagnetic radiation; fibroblasts; gel electrophoresis; genetic transcription; lymphocyte; messenger RNA; myelocyte; myeloid stem cell; neoplastic transformation; nucleic acid probes; phosphorylation; protooncogene; radiation carcinogenesis; simian virus 40; synchronous cell division; thymidine; tissue /cell culture

This research will investigate the proposed link between exposure of cells to extremely low frequency (ELF) electromagnetic (EM) fields and malignancy. We will determine if characteristics of neoplastic transformation and/or immortalization occur in human cells exposed to characterized ELF EM fields at 60 Hz. Aim 1. To determine if the expression of cellular proto-oncogenes is altered in human cells exposed to ELF EM fields by measurement of levels of mRNA transcripts and resulting proteins. Short exposures to defined ELF EM fields will be used initially; further experiments will determine how the time course or other parameters of exposure affect mRNA levels. Aim 2. To determine if cell divisional rates are changed by EM field exposure. Aim 3. To investigate the role of signal transduction in cell response to ELF EM fields; can a relation with signal transduction be made. as predicted on the basis of previous studies? Our major aim is to obtain adequate background information to ultimately allow us to experimentally test whether ELF EM field exposure can play a role in initiating, or augmenting neoplastic disease. The ramifications of this research, however, have bearing on the general safety of exposure to EM fields in industrial and everyday life.

这项研究将调查细胞暴露之间提出的联系 到极低的频率（ELF）电磁（EM）和 恶性。 我们将确定肿瘤的特征是否 转化和/或永生化发生在暴露于 以60 Hz为特征的Elf EM领域。 目标1。确定是否 在人类细胞暴露的人类细胞中，细胞原始基因的表达改变了 通过测量mRNA转录的水平和结果 蛋白质。 最初将使用对定义的ELF EM领域的短曝光； 进一步的实验将确定时间课程或其他参数 暴露会影响mRNA水平。 目标2。确定细胞分区是否 通过EM现场暴露更改费率。 目标3。调查角色 细胞对ELF EM场的反应中的信号转导；可以与 进行信号转导。正如先前的研究所预测的吗？ 我们的主要目的是获取足够的背景信息以最终 允许我们通过实验测试Elf em田间暴露是否可以玩 在启动或增强肿瘤疾病中的作用。 后果 但是，这项研究与暴露的一般安全有关 在工业和日常生活中的领域。