TGF-B SUPERFAMILY AND MOUSE DEVELOPMENT

TGF-B 超家族和小鼠发育

• 批准号: 2888702
• 负责人: Chester W Brown
• 金额: $ 8.1万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2888702
• 关键词: bone development; embryogenesis; embryonic stem cell; gene expression; gene targeting; genetic strain; genetic transcription; in situ hybridization; inhibin; laboratory mouse; mutant; northern blottings; protein structure function; southern blotting; species difference; transforming growth factors

DESCRIPTION: (Adapted from applicant's abstract) Growth and differentiation factor 3/Vg-1-related protein (GDF-3/Vgr-2), activin BA and activin BB are members of the transforming growth factor beta superfamily. Members of this superfamily play important roles in a variety of developmental processes. In an effort to aid in the characterization of proteins from this superfamily, the candidate intends to study the functions of GDF-3/Vgr-2 and the activins using the following strategies: In phase I of this proposal the aims are: (1) To further characterize the GDF-3/Vgr-2 gene by studying the pattern of transcription from this gene, and to determine the conservation of this gene among species; (2) To analyze the expression pattern of GDF-3/Vgr-2 mRNA in the developing mouse embryo and in adult mouse tissues by in situ hybridization; (3) To inactivate the GDF-3/Vgr-2 gene by homologous recombination in embryonic stem cells. (4) To substitute the activin BA mature region with activin BB to determine if these proteins are functionally interchangeable. In phase II of this proposal the aims are: (1) To continue to characterize the morphologic, histologic and biochemical characteristics of the mutant mice produced during phase 1; and (2) To crossbreed mice carrying a mutation in the GDF-3/Vgr-2 locus with other mice deficient in bone morphogenetic proteins and activins.

描述：（改编自申请人的摘要）生长和分化 因子 3/Vg-1 相关蛋白 (GDF-3/Vgr-2)、激活素 BA 和激活素 BB 是 转化生长因子β超家族的成员。 本会成员 超家族在多种发育过程中发挥着重要作用。 为了帮助鉴定蛋白质的特性 超家族，候选人打算研究GDF-3/Vgr-2的功能和 激活素使用以下策略： 本提案第一阶段的目标是： (1) 进一步描述 GDF-3/Vgr-2基因通过研究该基因的转录模式， 并确定该基因在物种间的保守性； (2) 分析 发育中的小鼠胚胎中 GDF-3/Vgr-2 mRNA 的表达模式 以及通过原位杂交在成年小鼠组织中进行； (3) 停用 GDF-3/Vgr-2 基因在胚胎干细胞中通过同源重组。 (4) 用激活素 BB 替代激活素 BA 成熟区以确定是否 这些蛋白质在功能上是可以互换的。 在本次第二阶段 提案的目标是：（1）继续表征形态， 产生的突变小鼠的组织学和生化特征 在第一阶段； (2) 杂交携带基因突变的小鼠 GDF-3/Vgr-2 基因座与其他骨形态发生蛋白缺陷小鼠 和激活素。