Enhancing the yield of industrial Actinomycete fermentations

提高工业放线菌发酵的产量

• 批准号: BB/N023544/1
• 负责人: Paul Hoskisson
• 金额: $ 88.78万
• 依托单位: University of Strathclyde
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FN023544%2F1
• 关键词: Enhancing; yield; industrial; Actinomycete; fermentations

A new and radical approach to strain construction is needed for the UK to remain competitive in manufacturing off-patent antibiotics. Antibiotic fermentation processes balance nutrients and cell growth rate with the chemical and physical environments. Cellular output is traditionally improved by selecting higher-producing mutants and optimising fermentation media. However, many antibiotics are unstable in the extracellular environment, with well characterised relationships between pH and degradation rate. The pH range for operation of the biological system often conflicts with the optimum for chemical stability of the product. For an established commercial process we propose to utilise recent advances in synthetic biology and genomics to develop a bacterial strain and industrial fermentation process capable of operating at a lower pH, facilitating stability of the desired product.

英国需要一种新的、激进的菌株构建方法，以在制造非专利抗生素方面保持竞争力。抗生素发酵过程平衡营养物质和细胞生长速度与化学和物理环境。传统上，通过选择产量更高的突变体和优化发酵培养基来提高细胞产量。然而，许多抗生素在细胞外环境中不稳定，pH 值和降解率之间的关系具有明确的特征。生物系统运行的 pH 范围常常与产品化学稳定性的最佳值相冲突。对于已建立的商业工艺，我们建议利用合成生物学和基因组学的最新进展来开发能够在较低 pH 下运行的细菌菌株和工业发酵工艺，从而促进所需产品的稳定性。

项目成果

Evolutionary dynamics of natural product biosynthesis in bacteria.

细菌天然产物生物合成的进化动力学。

• DOI: 10.1039/c9np00048h
• 发表时间: 2019-12-11
• 期刊: Natural product reports
• 影响因子: 11.9
• 作者: M. Chevrette;K. Gutiérrez;Nelly Sélem;César Aguilar;A. Yañez;Hilda E Ramos;P. Hoskisson;F. Barona
• 通讯作者: F. Barona

ActDES - a curated Actinobacterial Database for Evolutionary Studies.

ActDES - 用于进化研究的精选放线菌数据库。

• DOI: http://dx.10.1099/mgen.0.000498
• 发表时间: 2021
• 期刊: Microbial genomics
• 影响因子: 3.9
• 作者: Schniete JK

ActinoBase: tools and protocols for researchers working on Streptomyces and other filamentous actinobacteria

ActinoBase：为研究链霉菌和其他丝状放线菌的研究人员提供的工具和协议

• DOI: http://dx.10.1099/mgen.0.000824
• 发表时间: 2022
• 期刊: Microbial Genomics
• 影响因子: 3.9
• 作者: Feeney M

ActDES - a Curated Actinobacterial Database for Evolutionary Studies

ActDES - 用于进化研究的精选放线菌数据库

• DOI: http://dx.10.1101/2020.05.20.105536
• 发表时间: 2020
• 作者: Schniete J

Regulation of specialised metabolites in Actinobacteria - expanding the paradigms.

放线菌中专门代谢物的调节——扩展范式。

• DOI: http://dx.10.1111/1758-2229.12629
• 发表时间: 2018
• 期刊: Environmental microbiology reports
• 影响因子: 3.3
• 作者: Hoskisson PA