Optimisation of sortase-mediated protein labelling as a tool for biotechnology and pharmaceutical development

优化分选酶介导的蛋白质标记作为生物技术和药物开发的工具

• 批准号: BB/R005540/1
• 负责人: Michael Webb
• 金额: $ 27.04万
• 依托单位: University of Leeds
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FR005540%2F1
• 关键词: Optimisation; sortase; mediated; protein; labelling; Optimisation; sortase; mediated; protein; labelling

Labelling of proteins is fundamental to laboratory investigations and industrial applications such as the development of diagnostics. The market for protein labelling reagents is worth approximately $2bn a year and new innovations which increase the efficiency of protein labelling and reduce the cost of this process are always needed. In this project, we are developing a new protocol for the use of sortase in protein labelling. This technology has the potential to allow the rapid (<1 hour) generation of homogenously modified proteins with minimal excess reagent and small quantities of the labelling protein. During the project we will investigate a range of potential commercial applications including the potential of the technology for application in the generation of modified antibodies, which represent a major new class of anti-cancer drugs.

蛋白质的标记是实验室研究和工业应用的基础，例如诊断的开发。蛋白质标签试剂的市场每年价值约200亿美元，新的创新提高了蛋白质标签的效率并始终需要降低此过程的成本。在这个项目中，我们正在开发一种用于在蛋白质标记中使用分子酶的新协议。这项技术有可能允许快速（<1小时）生成同质修饰的蛋白质，具有最小的过量试剂和少量的标记蛋白。在该项目期间，我们将研究一系列潜在的商业应用，包括该技术在修改后的生成中应用的潜力，这些抗体代表了一类新的抗癌药物。