OSCILLATIONS AND THE CONTROL OF GLYCOLYSIS

振荡和糖酵解的控制

• 批准号: 3230164
• 负责人: KEITH TORNHEIM
• 金额: $ 12.79万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-04-01 至 1989-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3230164
• 关键词: 6 phosphofructokinase; adenosine diphosphate; adenosine triphosphate; bioenergetics; carbohydrate metabolism; cell free system; citrates; creatine phosphate; fluorimetry; fructose phosphate; glucose phosphate; glyceraldehyde 3 phosphate dehydrogenase; glycolysis; muscle metabolism; phosphorylases; spectrometry; striated muscles; tissue /cell culture; vascular smooth muscle

Oscillatory behavior of glycolysis is observed when cell-free extracts of rat skeletal muscle are provided with glucose. Experimental studies and theoretical treatments have indicated that under certain conditions such oscillatory behavior can maintain a higher [ATP]/[ADP] ratio than corresponding steady state or steady flux behavior. This project will examine the effects of fructose 2,6-bisphosphate, citrate creatine phosphate and orthophosphate, in comparison with roles suggested by models of the oscillations. The importance of the level of glyceraldehyde 3-phosphate dehydrogenase activity in determining oscillatory or steady state behavior will be tested, as will the possibilities for interacting control of phosphofructokinase and phosphorylase with glycogen as carbon source. Spectrophotometric and fluorometric techniques will be used for continuously monitoring the system, and frequent samples will be assayed for all the glycolytic intermediates in order to follow in detail the changing pattern of the glycolytic flux under different conditions. The objective of this project is to clarify the role of specific control properties of the individual enzymes in determining the behavior of the system as a whole and to relate the complex behavior to appropriate metabolic contexts. A second objective is to obtain evidence for the occurrence of such oscillations in muscle in vivo, using very thin intact muscle and also cultured chick myotubes and rat aortic smooth muscle cells; spectrophotometric and fluorometric techniques will be used, together with metabolite assays where possible.

当无细胞提取物的提取物的振荡行为会观察到 大鼠骨骼肌配有葡萄糖。 实验研究和 理论治疗表明，在某些条件下 振荡行为可以维持更高的[ATP]/[ADP]比 相应的稳态或稳定的通量行为。 这个项目将 检查果糖2,6-双磷酸盐，柠檬酸肌酸的作用 与模型所建议的角色相比 振荡。 甘油醛水平的重要性 3-磷酸​​盐脱氢酶在确定振荡或稳定方面的活性 状态行为将进行测试，互动的可能性也将 用糖原作为碳的磷酸果糖激酶和磷酸化酶的控制 来源。 分光光度法和荧光测量技术将用于 连续监视系统，并将测定频繁的样品 对于所有糖酵解中间体，以详细遵循 在不同条件下变化的糖酵解通量的模式。 这 该项目的目的是阐明特定控制的作用 单个酶的特性在确定的行为中 整个系统，并将复杂行为与适当的行为联系起来 代谢上下文。 第二个目标是获得证据 使用非常薄的肌肉在体内发生这种振荡 肌肉以及培养的雏鸡肌管和大鼠主动脉平滑肌细胞； 分光光度法和荧光测量技术将与 代谢物测定法。