STUDY OF A LOW-POTENTIAL ELECTROCHEMICAL GLUCOSE SENSOR

低电位电化学葡萄糖传感器的研究

• 批准号: 3231057
• 负责人: SHANG J YAO
• 金额: $ 10.74万
• 依托单位: MONTEFIORE UNIVERSITY HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-12-01 至 1988-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3231057
• 关键词: artificial endocrine pancreas; atomic absorption spectrometry; blood chemistry; blood glucose; clinical biomedical equipment; electrical potential; electrochemistry; gas chromatography; human tissue; implant; insulin; mathematical model; microelectrodes; potentiometry; silver chloride electrode; urinalysis

The goal is the development of an electrochemical glucose sensor which is capable of being implanted into the vascular or extracellular space of a diabetic patient for continuous in vivo monitoring of his blood sugar level. Its use would eliminate the inconvenience of intermittent withdrawal of blood for glucose assay, as well as the control lag which this imposes. A direct bedside readout would benefit the acute case of diabetes and such a sensor, when coupled with an insulin infusion pump, and interfaced with a feedback system, would complete the development of an integrated artificial endocrine pancreas. A number of pumps and feedback systems are currently under clinical evaluation in the United States. Central to the artificial pancreas is the glucose sensor which is far behind the other components in its development. The availability of such a sensor would constitute a breakthrough for both clinical practice and basic investigation of diabetes and its associated complications. Potentiodynamic studies have revealed two new distinct low-potential redox peaks at the smooth Pt electrode that can be utilized for glucose monitoring even in the ultrafiltrate of human serum. This reaction is reproducible since the electrode surface is regenerated by electrochemical pulsing. The two peaks are located at well-defined potentials: -0.72 V (anodic oxidation) and -0.80 V (cathodic reduction) vs Ag/AgCl. These potentials are stable and do not shift with increasing glucose concentration. The in vitro study has shown that an electrode using this reaction should be operable at glucose levels up to 400 mg/dl. The specific aims are: (1) to construct a standard, controlled geometry test electrode using thick film deposition technique; (20 to use this electrode in parametric tests to characterize the mechanism by which the redox peaks are generated; (3) to select the proper voltammetric conditions for the practical development of the sensor; (4) to evaluate the specificity of the peaks for glucose in the presence of potentially interfering substances which might be present in human blood and tissue fluid; (5) to determine if trace amounts of Pat and Ag are released during prolonged voltammetry; (6) to design and construct a miniaturized, implantable electrode array using thin film deposition techniques.

目标是开发电化学葡萄糖传感器 能够被植入到血管或细胞外空间 糖尿病患者连续体内血糖监测 等级。 它的使用将消除间歇性的不便 抽血进行葡萄糖测定，以及控制滞后 这强加了。 直接床边读数将有利于急性病例 糖尿病和这样的传感器，当与胰岛素输注泵结合使用时，以及 与反馈系统接口，将完成一个 集成人工内分泌胰腺。 多个泵和反馈 该系统目前正在美国进行临床评估。 人工胰腺的核心是葡萄糖传感器，它距离 落后于其他组件的开发。 这样的可用性 传感器将构成临床实践和基础的突破 糖尿病及其相关并发症的调查。 动电位研究揭示了两种新的独特的低电位氧化还原 光滑 Pt 电极处的峰值可用于葡萄糖 甚至可以在人血清的超滤液中进行监测。 这个反应是 由于电极表面通过电化学再生，因此可重复 脉动。 两个峰值位于明确的电位处：-0.72 V （阳极氧化）和 -0.80 V（阴极还原）相对于 Ag/AgCl。 这些 电位稳定，不会随着葡萄糖的增加而变化 专注。 体外研究表明，使用这种电极 反应应在高达 400 mg/dl 的葡萄糖水平下进行。 具体目标是：（1）构建标准的、受控的几何结构 采用厚膜沉积技术的测试电极； （20 使用此 参数测试中的电极来表征其机制 产生氧化还原峰； (3)选择合适的伏安条件 用于传感器的实际开发； (4) 评估 存在潜在的葡萄糖峰的特异性 人体血液和组织中可能存在的干扰物质 体液; (5) 测定过程中是否释放微量Pat和Ag 长时间伏安法； (6) 设计和建造小型化、 使用薄膜沉积技术的植入式电极阵列。