IMMUNOLOGIC ACTIVITY OF MALLORY BODIES IN LIVER DISEASE

肝脏疾病中马洛里体的免疫活性

• 批准号: 3232919
• 负责人: CARROLL B LEEVY
• 金额: $ 11.79万
• 依托单位: UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-23 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3232919
• 关键词: alcoholic hepatitis; alcoholism /alcohol abuse; antibody specificity; autoradiography; biliary tract disorder; drug related neoplasm /cancer; gel electrophoresis; hepatocellular carcinoma; human tissue; humoral immunity; hyaline substance; liver disorder diagnosis; lymphokines; monoclonal antibody; radioimmunoassay; radiotracer; serology /serodiagnosis; surface antigens

This research is based on the development of a specific monoclonal antibody (anti NMB-1) to purified isolates of Mallory body. The anti-NMB-1 will be used to develop a sensitive and specific immunoassay to detect Mallory body antigen and its antibody in sera of patients with hepatic disorders containing Mallory bodies. The usefulness of such an assay as a diagnostic, therapeutic and prognostic guide will be explored using double blind prospective studies on serum from patients with various hepatic disorders with and without Mallory bodies. Anti-NMB-1 will be utilized as a probe to explore the cytoplasmic components that lead to the formation of Mallory body and its relation to hepatocellular cancer following administration of griseofulvin. The antigenic moiety of Mallory body will be identified by transblotting technique using MBAb positive sera. The physicochemical nature of the isolated antigenic moiety of Mallory body will then be studied. Chemical composition of antigenic body isolates will be determined and additional monoclonal antibodies developed from isolates of Mallory body from alcoholic hepatitis, primary biliary cirrhosis and hepatocellular cancer. Finally, the relationship of clinical and functional status, and the influence of treatment on MBAg and MBAb will be assessed.

这项研究基于特定单克隆抗体的开发 （抗NMB-1）纯化的马洛里人的分离株。 抗NMB-1将是 用于开发敏感且特定的免疫测定法以检测Mallory身体 抗原及其抗体抗体的肝疾病患者 包含马洛里的身体。 这样的测定法 诊断，治疗和预后指南将使用双重探索 盲目研究各种肝的患者的血清 有或没有马洛里身体的疾病。 抗NMB-1将被用作 探索探索导致形成的细胞质成分 马洛里的身体及其与肝细胞癌的关系 磨牙的管理。 马洛里身体的抗原部分将 通过使用MBAB阳性血清来鉴定通过发射技术。 这 马洛里身体孤立的抗原部分的物理化学性质 然后将研究。 抗原体分离株的化学组成将 可以确定并从分离株中发展出其他单克隆抗体 酒精肝炎，原发性胆汁肝硬化和 肝细胞癌。 最后，临床和 功能状态，治疗对MBAG和MBAB的影响将是 评估。