BIOCHEMICAL STUDIES OF MINERALIZED TISSUE PROTEOGLYCANS

矿化组织蛋白聚糖的生物化学研究

• 批准号: 3220236
• 负责人: CHARLES W PRINCE
• 金额: $ 10.93万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-05-01 至 1994-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3220236
• 关键词: 1,25 dihydroxycholecalciferol; bone metabolism; calcification; cell bank /registry; chemical structure function; connective tissue metabolism; dentin; glycoproteins; laboratory rabbit; laboratory rat; macromolecule; normal ossification; proteoglycan; proteolysis; radionuclides; radiotracer; retinoids; sialate; sulfur

The major hypothesis upon which this research is based is that non-collagenous components of mineralized connective tissues are intimately involved in the formation of the tissues. Furthermore, an understanding of the chemical composition, localization and regulatory mechanisms of biosynthesis are required before specific, functional roles can be elucidated. In this proposal we will address the aforementioned areas as they relate to proteoglycans and osteopontin, newly discovered bone-derived cell attachment protein. The approach taken is to isolate chemical amounts of these components, verify their purity and use them as antigens. The antibodies thus produced will be used in immunolocalization studies and as reagents in immunoassays. We will explore in detail the kinetics of proteoglycan biosynthesis in rat calvaria and long bone, in ROS 17/2.8 cells and in cultures of collagenase-derived rat calvarial osteoblasts. We will evaluate the role and mechanism that 1,25-dihydroxyvitamin D3 and parathyroid may have in the regulation of proteoglycan and osteopontin biosynthesis. We will also further explore the mechanism of action by which osteopontin stimulates cells to attach in vitro.

这项研究基于的主要假设是 矿化结缔组织的非胶原成分是 密切参与组织的形成。 此外， 了解化学组成，定位和 需要生物合成的调节机制 可以阐明特定的功能作用。 在这个建议中，我们 将解决与上述区域有关的问题 蛋白聚糖和骨桥，新发现的骨源性细胞 附着蛋白。 采用的方法是隔离化学 这些组件的数量，验证它们的纯度并将其用作 抗原。 这样生产的抗体将用于 免疫定位研究和作为免疫测定中的试剂。 我们 将详细探讨蛋白聚糖生物合成的动力学 Ros 17/2.8细胞中的大鼠瓦尔瓦里亚和长骨 胶原酶衍生的大鼠钙钙细胞。 我们将评估 1,25-二羟基维生素D3和 甲状旁腺可能在蛋白聚糖的调节中具有 骨桥蛋白生物合成。 我们还将进一步探索 骨桥蛋白刺激细胞的作用机理 体外附着。