HIV INHIBITORY FACTORS IN HUMAN SALIVA

人类唾液中的艾滋病毒抑制因子

• 批准号: 3223356
• 负责人: Daniel Malamud
• 金额: $ 25.67万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-06-04 至 1993-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3223356
• 关键词: antiAIDS agent; antiviral agents; electron microscopy; genetic library; genetic manipulation; human immunodeficiency virus; human subject; molecular cloning; parotid gland; protein purification; protein sequence; protein structure; saliva; salivary disorder; submandibular gland; virus protein

Epidemiologic evidence indicates that transmission of HIV by the oral route occurs infrequently, if at all. Laboratory studies by others, and our own preliminary results presented in this proposal, have detected a factor in saliva which inhibits HIV infectivity. The factor(s) appears to be protein, are present in highest concentration in submandibular saliva, and result in a loss of viral infectivity in vitro. In this reaction, two salivary proteins (MW 32,000 and 35,000 daltons) appear to become associated with HIV aggregates. The aims of the present study are to characterize the interaction between the salivary factor and HIV and to purify the protein from normal human submandibular saliva. Antibody or oligomer probes would be generated and used to screen a submandibular gland cDNA library to identify clones expressing the recombinant protein. The purified salivary protein, or the recombinant molecule, would then be used to study the mechanism of interaction with HIV protein(s). Finally, the level of anti-HIV activity in saliva of normal individuals and subjects with xerostomia will be evaluated to determine if decreased salivary flow is associated with a decreased amount of anti-HIV activity. These studies would provide the basis for understanding the role of saliva in modulating HIV transmission. Subsequent studies would investigate the HIV seropositive and AIDS populations to determine if the activity is altered after virus infection.

流行病学证据表明，口腔途径传播HIV 如果有的话，很少发生。 他人和我们自己的实验室研究 本提案中提出的初步结果已检测到一个因素 抑制HIV感染性的唾液。 因子似乎是 蛋白质存在于下颌下唾液中的最高浓度，并且 导致体外病毒感染性的丧失。 在这个反应中，两个 唾液蛋白（MW 32,000和35,000 daltons）似乎已成为 与HIV骨料有关。 本研究的目的是 表征唾液因子与艾滋病毒与艾滋病毒之间的相互作用 从正常的人类下唾液中纯化蛋白质。 抗体或 将生成低聚物探针并用于筛选下颌腺 cDNA文库以识别表达重组蛋白的克隆。 这 然后将使用纯化的唾液蛋白或重组分子 研究与HIV蛋白相互作用的机制。 最后， 正常人和受试者唾液中抗HIV活性水平 将评估静态症，以确定唾液流量减少是否减少 与抗HIV活性量减少有关。 这些研究将为理解唾液的作用提供基础 在调节HIV传播中。 随后的研究将研究 HIV血清阳性和援助人群来确定活动是否是 病毒感染后改变。