ULTRASTRUCTURE OF EXPERIMENTAL PERIODONTITIS

实验性牙周炎的超微结构

• 批准号: 3218927
• 负责人: PHILIAS R. GARANT
• 金额: $ 13.98万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-06-01 至 1990-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3218927
• 关键词: Actinomyces; Actinomycetales infection; Fusobacterium; antibody formation; autoradiography; bacterial antigens; cellular pathology; colchicine; collagen; communicable disease diagnosis; concanavalin A; dental disorder diagnosis; dental plaque; dogs; endoplasmic reticulum; fibroblasts; fibronectins; freeze etching; germ free condition; gingiva; gram negative bacteria; gram positive bacteria; histochemistry /cytochemistry; histopathology; immunocytochemistry; immunoelectron microscopy; intermediate filaments; laboratory rabbit; laboratory rat; ligaments; lymph nodes; lysosomes; macrophage; microfilaments; mitochondria; monocyte; oral bacteria; oral mucosa; osteoclasts; parotid gland; periodontitis; periodontium; peroxidases; phagocytosis; radiotracer; scanning electron microscopy; serology /serodiagnosis; surface antigens; tissue /cell culture; tooth resorption; tritium

We plan to conduct immunocytochemical studies to localize fibronectin (FN) and collagen types I and III (Col I, Col III) in normal beagle periodontium and to determine changes in their distribution during ligature-induced and naturally-occurring periodontitis. We will measure FN, Col I, and Col III in gingival sulcular fluid and correlate these levels with inflammation as determined by light and electron microscopy and immunocytochemistry. Using the same methods, we will study the effect of inhibiting collagenase with minocycline on the level of FN, Col I, and Col III in gingival sulcular fluid. We expect to show that monitoring FN, Col I, and Col III in gingival crevicular fluid can be an accurate way to measure ongoing tissue destruction. If the beagle studies prove encouraging, we will begin similar investigations in humans. In a related project, we will use immunocytochemical methods to investigate the presence of FN in gingival plaque and calculus. We will continue autoradiographic studies of the penetration and incorporation into gingival epithelial cells of small metabolites applied to the gingival sulcular area. We hope to determine whether cell-to-cell communication and intracytoplasmic transport is a significant pathway in gingival epithelium. Preliminary findings suggest that further studies appear worthwhile. During the tenure of this grant, we have conducted numerous and basic studies of collagen secretion and fibroblast structure. We propose to continue these important studies by localizing fibroblasts rich in stress fibers by fluorescent microscopy with anti-actin and intermediate filaments with anti-vimentin antibodies. We will also examine the distribution of FN in relation to the development of fibronexus contacts by electron microscopy immunocytochemistry in areas previously identified by light microscopy to be sites rich in stress fiber development. We hope to correlate their presence with resistance to stress in the periodontal connective tissues. In vitro studies are also planned to further evaluate the polarity and locomotion of periodontal ligament fibroblasts in relation to the presence and distribution of stress fibers and fibronectin.

我们计划进行免疫细胞化学研究以定位纤连蛋白（FN） I和III型胶原蛋白（Col I，Col III）在正常Beagle牙周上 并确定其在结扎诱导的过程中分布的变化和 天然牙周炎。 我们将测量FN，Col I和Col III 在牙龈胰岛流体中，并将​​这些水平与炎症相关 由光和电子显微镜和免疫细胞化学确定。 使用 同样的方法，我们将研究抑制胶原酶与 在牙龈硫的FN，Col I和Col III水平上的米诺环素 体液。 我们希望在 牙龈裂缝液可以是测量正在进行组织的准确方法 破坏。 如果比犬研究证明令人鼓舞，我们将开始 在人类中进行了类似的调查。 在一个相关项目中，我们将使用 免疫细胞化学方法研究牙龈中FN的存在 斑块和微积分。 我们将继续对渗透和 掺入小型代谢物的牙龈上皮细胞中 到牙龈胰岛区域。 我们希望确定是否细胞到细胞 通信和胞质内运输是一个重要的途径 牙龈上皮。 初步发现表明进一步研究 看起来值得。 在这笔赠款期间，我们进行了 胶原蛋白分泌和成纤维细胞结构的大量基础研究。 我们建议通过定位成纤维细胞来继续这些重要研究 通过抗肌动蛋白和荧光显微镜富含应力纤维 抗Vimentin抗体的中间细丝。 我们还将检查 FN与Fibronexus的发展有关 以前通过电子显微镜免疫细胞化学接触 通过光学显微镜确定为富含应力纤维的位点 发展。 我们希望将它们的存在与抵抗压力相关联 在牙周结缔组织中。 还计划了体外研究 进一步评估牙周韧带的极性和运动 与应力纤维的存在和分布有关的成纤维细胞 和纤连蛋白。