NEUTROPHIL FUNCTION IN PERIODONTAL DISEASE

牙周疾病中的中性粒细胞功能

基本信息

批准号：
3221596
负责人：
ROLAND R ARNOLD
金额：
$ 22.76万
依托单位：
EMORY UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1987
资助国家：
美国
起止时间：
1987-09-01 至 1990-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3221596
关键词：
Actinobacillus Bacteroides Escherichia coli Staphylococcus aureus Streptococcus mutans adolescence (12-20) chemotaxis human subject immunoglobulin A laboratory rabbit ligands membrane structure microorganism immunology neutrophil oral bacteria periodontium disorder saliva secretory immune system

项目摘要

Actinobacillus actinomycetemcomitans (Aa) and Bacteroides gingivalis (Bg) have implicated in the etiology of certain forms of periodontal disease. It has been suggested that the neutrophil plays a primary role in the defense against establishment of these purported periodontopathogens. The overall objective of this proposed work is to define the mechanisms of the PMN involved in the defense against periodontal diseases by examining and defining the functional defects of patients with periodontal disease. It is well established that the PMN of patients with localized juvenile periodontitis (LJP) have quantitative chemotactic defects. Preliminary studies with LJP PMN suggest they are capable of normal phagocytoses of Aa; however, they fail to kill these organisms. These studies will, therefore, examine the LJP PMN to determine the underlying dysfunction that results in this inability to kill Aa. It will first be determined if this dysfunction is specific or a generalized deficiency in killing of all microorganisms by examining LJP PMN of their ability to kill not only Aa, but also Bg, Staphylococcus aureus, Escherichia coli, and Streptococcus mutans. The bactericidal activity of the LJP PMN will be compared with that of healthy controls, and of patients with chronic adult or refractory periodontitis in the presence and absence of patient and control sera and saliva. Recent studies indicate that there are distinct subsets of PMN granules that may constitute functionally distinct populations. A quantitative or qualitative defect in one or more of these granule subsets may result in an inability to kill a specific microoganisms or group of microoganisms. The granule profiles obtained by isopycnic gradient centrifugation of fractions of patient and control PMN will be compared using biochemical analyses for selected granule markers. In addition, the granule content and supernatants of the various cell populations will be examined following stimulation with opsonized test organisms or specific secretogogues. It is further proposed to study the functional abilities of 1gA-Fc receptor positive subpopulation of PMN and their influence by serum and secretory antibodies. The surface characteristics of 1gA+ and 1gA- PMNs will be characterized using other ligands as probes of membrane structure by direct binding assays. A study of these patient populations offers a unique opportunity to dissect the functional roles of subsets of PMN granules and enzymes and subset populations of cells in relation to susceptibility to specific periodontopathogens.

放线杆菌放线菌菌（AA）和菌落牙龈（BG）与某些形式的病因有关牙周疾病。有人建议中性粒细胞在防御这些建立方面起着主要作用据称牙周病。总体目标建议的工作是定义涉及的PMN的机制通过检查和定义牙周患者的功能缺陷疾病。众所周知，患者的PMN 局部少年牙周炎（LJP）具有定量趋化性缺陷。 LJP PMN的初步研究表明他们能够正常的AA吞噬作用；但是，他们失败了杀死这些生物。因此，这些研究将检查 LJP PMN确定结果的潜在功能障碍为了杀死AA。如果这个将首先确定功能障碍是特定的或杀死所有功能的普遍缺陷微生物通过检查LJP PMN的杀戮能力只有AA，但BG，金黄色葡萄球菌，大肠杆菌和链球菌突变。 LJP PMN的杀菌活性将与健康对照组和患者进行比较在存在的慢性成人或难治性牙周炎中缺乏患者和控制血清和唾液。最近的研究表明有不同的PMN颗粒子集可能构成功能不同的人群。定量或这些颗粒子集中一个或多个的定性缺陷可能导致无法杀死特定的微型人或一组微型人性。通过等光谱获得的颗粒剖面患者和对照PMN的分数的梯度离心将使用选定颗粒的生化分析进行比较标记。另外，颗粒含量和上清液刺激后将检查各种细胞种群带有调子测试生物或特定的秘诀。这是进一步研究1GA-FC的功能能力受体对PMN的阳性亚群及其影响血清和分泌抗体。表面特征 1GA+和1GA- PMN将使用其他配体来表征通过直接结合测定法进行膜结构探针。一项研究这些患者人口提供了独特的机会来剖析 PMN颗粒和酶的子集的功能作用以及细胞的子集与特定的敏感性有关牙周病。