MECHANISMS OF FLUORIDE ACTION:IN VITRO & IN VIVO STUDIES

氟化物作用机制：体外

• 批准号: 3219074
• 负责人: JAMES STERN WEFEL
• 金额: $ 19.78万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-07-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3219074
• 关键词: X ray crystallography; acidity /alkalinity; calcification; calcification stimulant; chemical models; chemical structure function; dental caries inhibitor; fluoridation; human subject; saliva

The process of dental decay is a dynamic one involving episodes of demineralization and remineralization, and may be prevented by the proper use of fluoride. The overall objectives are to increase our understanding of demineralization and remineralization processes in order to develop clinically applicable remineralization fluids and to optimize use of existing preventive agents. The mechanisms of action of fluoride which are the primary focus of this grant are the inhibition of demineralization and the enhancement of remineralization. These two mechanisms will be investigated both in vitro and in vivo using enamel surfaces. The specific aims are to test the hypothesis that acidic remineralizing fluids are more effective than neutral fluids on either surface softened or subsurface lesions; to test whether these remineralized lesions will be more resistant to subsequent acid attacks; to use in vitro pH cycling to test the ability of remineralizing fluids to prevent, arrest, and/or repair lesions; and to use an intraoral system to test the ability of remineralizing agents to enhance the ability of saliva to prevent, arrest, and/or repair lesions. The remineralization experiments will be conducted under relatively constant ion composition and will be followed kinetically. The amount, site and type of remineralization will be determined by polarized light microscopy and quantitative microradiography on the single sections. Both SEM with energy dispersive analysis nad x-ray diffraction of the lesion mineral will be used to evaluate the nature of the remineralized lesion, the size of the crystals and gross morphology. The episodic nature of dental caries will be studied using a pH cycling model in vitro and an intra-oral model in vivo. The in vivo experiments will rely on saliva as the main remineralizing agent, while test fluids will be used to "prime" the hard tissue and enhance the effectiveness of saliva. Thus, techniques for prevention and repair of enamel caries will be developed, tested and made clinically applicable.

牙齿衰变的过程是一个动态的，涉及的情节 脱矿和回忆，可以通过适当的 使用氟化物。 总体目标是增加我们的理解 为了发展的矿化和回忆过程 临床适用的回忆流体，并优化 现有的预防剂。 氟化物的作用机理 这笔赠款的主要重点是抑制脱矿化和 增强回忆性。 这两个机制将是 使用搪瓷表面研究了体外和体内。 具体目的是检验酸性回忆化的假设 流体比表面软化或中性流体更有效 地下病变；测试这些回忆的病变是否会 对随后的酸攻击具有更大的抵抗力；使用体外pH循环到 测试回忆流体预防，逮捕和/或维修的能力 病变；并使用口腔内系统测试 回忆剂以增强唾液预防，逮捕， 和/或修复病变。 回忆实验将在相对 恒定的离子组成，并将按动力学遵循。 量， 地点和回忆的类型将通过极化光确定 单切片上的显微镜和定量显微放射图。 两个都 SEM带有能量分散分析NAD X射线病变的NAD X射线衍射 矿物将用于评估回忆性病变的性质， 晶体的大小和总形态。 情节性质 将在体外使用pH骑自行车模型研究龋齿 体内的口腔内模型。 体内实验将依靠唾液作为 主要回忆剂，而测试液将用于“素数” 硬组织并增强唾液的有效性。 因此，技术 为了预防和维修搪瓷龋齿的开发，测试和 使临床适用。