MECHANISMS OF SUGAR TRANSPORT IN STREPTOCOCCUS MUTANS

变形链球菌的糖转运机制

• 批准号: 3219677
• 负责人: GARY R JACOBSON
• 金额: $ 7.2万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-06-01 至 1988-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3219677
• 关键词: Streptococcus mutans; carbohydrate metabolism; carbohydrate transport; dental caries; enzyme structure; glucose; microorganism culture; microorganism metabolism; oral bacteria; phosphorylation; phosphotransferases; scintillation counter; sucrose

The sucrose phosphotransferase system (PTS) is one means by which the oral pathogen Streptococcus mutans takes up this disaccharide from the environment. Because sucrose metabolism has been linked to the cariogenicity of S. mutans, our laboratory has been studying the biochemistry of the PTS in this organism. Our long-range goal is to understand the mechanism and regulation of the sucrose PTS in S. mutans to be able to identify important factors influencing the activity of this transport system in vivo. Our work also may lead to the identification of specific inhibitors of sucrose uptake and growth of S. mutans. In continuing this project, we aim to: 1) purify Enzyme I and the sucrose-specific enzyme III of the S. mutans PTS to apparent homogeneity and investigates their properties; 2) purify the sucrose-specific Enzyme II from S. mutans membranes; 3) further investigate sucrose analogs as inhibitors of the sucrose PTS and any other sucrose transport systems of S. mutans; 4) determine the physiological significance of ATP-dependent phosphyorylations of S. mutans proteins; and 5) continue 31p-NMR studies as a complementary approach toward understanding sugar transport systems and their regulation in S. mutans. A combination of well-accepted biochemical and biophysical approaches will be used to accomplish these aims.

蔗糖磷酸转移酶系统（PTS）是口服的一种手段。 病原体变形链球菌从 环境。 因为蔗糖代谢与 变形链球菌的致龋性，我们实验室一直在研究 该生物体中 PTS 的生物化学。 我们的长期目标是 了解变形链球菌中蔗糖 PTS 的机制和调节 能够识别影响该活动的重要因素 体内运输系统。 我们的工作还可能导致识别 蔗糖摄取和变形链球菌生长的特异性抑制剂。 在 继续这个项目，我们的目标是：1）纯化酶 I 和 变形链球菌 PTS 的蔗糖特异性酶 III 具有明显的同质性 并调查其属性； 2) 纯化蔗糖特异性酶 II 来自变形链球菌膜； 3) 进一步研究蔗糖类似物 蔗糖PTS和S.任何其他蔗糖转运系统的抑制剂。 突变体； 4）确定ATP依赖的生理意义 变形链球菌蛋白的磷酸化； 5) 继续 31p-NMR 研究 理解糖运输系统的补充方法和 它们在变形链球菌中的调节。 广泛接受的生化组合 生物物理方法将用于实现这些目标。