Production of D-lactate in Geobacillus spp App No 50484-338192

在地芽孢杆菌属中生产 D-乳酸 申请号 50484-338192

• 批准号: BB/M028674/1
• 负责人: David Jonathan Leak
• 金额: $ 10.08万
• 依托单位: University of Bath
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM028674%2F1
• 关键词: Production; lactate; Geobacillus; spp; App

Bioplastic, as a replacement for hydrocarbon plastic, is a rapidly expanding market that is predicted to grow from 1.4M Tonnes in 2012 to over 6M Tonnes by 2017 (Source: European Bioplastics). Within this market, Polylactic Acid (PLA) is an alternative material for existing products in packaging, food serviceware and textiles together with enabling new products to be developed for high value markets where desired variations in the properties of the PLA can be achieved by mixing of D and L isomers of lactic acid in different proportions. Current lactic acid production is mainly in the more common L isomer from 1st generation feedstocks, which compete with food crops for agricultural land and produce waste that requires processing prior to disposal. The D isomer is mainly produced from the L isomer through a combination of chemical and biological processes. This proposal seeks to develop a fully biological process to create an innovative and efficient manufacturing route for producing D lactic acid that reduces the dependency on fossil oil, leading to the de-carbonisation of the industrial process for the production of PLA. The collaboration brings together Rebio Technologies Ltd, the University of Bath and the Centre for Process Innovation (CPI). Rebio have expertise in the development of industrial strains based on a Geobacillus host for the production of 2nd generation bioethanol from a wide range of feedstocks such as cane bagasse, corn stover and fractions derived from municipal solid waste. This is combined with knowledge of the development of different biopolymers and their potential markets, and they are in a position to take a successful project forward into production. The University of Bath have considerable expertise in the genetic engineering of a wide range of organisms but specifically have a background in Geobacillus and will carry out the necessary strain engineering to convert an L-lactate to a D-lactate producing strain. CPI brings knowledge of the downstream processing of lactic acid and in the area of process scale up and will be able to evaluate market potential from data arising from from scale-up experiments .

作为碳氢化合物塑料的替代品，生物塑料是一个快速扩展的市场，预计将从2012年的140万吨增长到2017年的600万吨（来源：欧洲生物塑料）。在这个市场中，聚乳酸（PLA）是包装，食品服务软件和纺织品中现有产品的替代材料，并使其能够为高价值市场开发新产品，在高价值市场中，可以通过混合来实现PLA的所需变化。乳酸的D和L异构体不同比例。当前的乳酸产生主要是第一代原料中更常见的L异构体，这些原料与食品作物竞争农业土地，并产生需要处理前需要加工的废物。 d异构体主要是通过化学和生物过程组合从L异构体生产的。该提案旨在开发一个完全生物学的过程，以创建一种创新，高效的制造途径，以生产D乳酸，从而减少对化石油的依赖，从而导致生产PLA的工业过程去碳化。该合作汇集了Rebio Technologies Ltd，Bath大学和过程创新中心（CPI）。 REBIO在开发工业菌株方面具有专业知识，该菌株是基于地理位石宿主的，用于生产第二代Bioethanol，从甘蔗渣，玉米，玉米毒和从市政固体废物中得出的分数等广泛的原料。这与了解不同生物聚合物及其潜在市场的发展相结合，它们可以将成功的项目推进生产。巴斯大学在广泛的生物的基因工程方面具有丰富的专业知识，但具体具有地理位石背景，并将进行必要的应变工程，以将L-乳酸转化为D-乳酸产生菌株。 CPI带来了有关乳酸的下游加工以及在过程规模上的知识，并将能够评估来自扩大实验的数据的市场潜力。