EXPRESSION OF OFP DURING RAT MAMMARY CARCINOGENESIS

大鼠乳腺癌发生过程中 OFP 的表达

• 批准号: 3203037
• 负责人: THOMAS E WEBB
• 金额: $ 12.63万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-01-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3203037
• 关键词: autocrine; benzanthracenes; biomarker; breast neoplasms; carcinoembryonal antigen; cell growth regulation; chemical carcinogen; chemical carcinogenesis; chorionic gonadotropin; female; gene expression; glycoproteins; immunologic assay /test; laboratory rat; mammary gland; neoplastic cell; nucleic acid hybridization; oncoproteins; receptor binding; retinoids; tissue /cell culture

Based on N-terminal (amino acid) and C-terminal (base) sequences, OFP appears to be a novel protein. Normally present only in the fetus and placenta, it is re-induced early in carcinogenesis, persists in the tumor and is secreted to circulation. The hypothesis is that OFP is produced in and released from pre-malignant lesions in the rat mammary gland after treatment with 7,12-dimethylbenz(a)anthracene (DMBA). The DMBA-induced model will be used to identify mammary lesions producing OFP, and to correlate rate of production and steady state blood levels as a function of time with various histopathological parameters and tumorigenic potential. In addition to tumorigenesis modulating agents (i.e., retinoid and chorionic gonadotropin) this study will apply specific monoclonal antibodies and cDNA to an ELISA assay, immunolocalization and in situ hybridization. The possible interaction of OFP with the cell membrane (receptors) will be evaluated in rat mammary cell lines. The data from this study are relevant to detection of premalignant lesions, and to monitoring chemical carcinogenesis.

基于 N 端（氨基酸）和 C 端（碱基）序列，OFP 似乎是一种新型蛋白质。 通常仅存在于胎儿中 胎盘，在癌变早期被重新诱导，持续存在于肿瘤中 并分泌至循环系统。 假设 OFP 是产生的 在大鼠乳腺癌前病变中并释放后 用 7,12-二甲基苯并(a)蒽 (DMBA) 处理。 DMBA诱导的 模型将用于识别产生 OFP 的乳腺病变，并 将生产率和稳态血液水平作为函数关联起来 时间与各种组织病理学参数和致瘤性的关系 潜在的。 除了肿瘤发生调节剂（即， 视黄醇和绒毛膜促性腺激素）本研究将应用特定 单克隆抗体和 cDNA 用于 ELISA 测定、免疫定位和 原位杂交。 OFP 与细胞可能的相互作用 将在大鼠乳腺细胞系中评估膜（受体）。 这 这项研究的数据与癌前病变的检测相关， 并监测化学致癌作用。