Glyconanoparticle diagnostics for the detection and discrimation of human and avian influenza infection

用于检测和区分人类和禽流感感染的糖纳米颗粒诊断

• 批准号: BB/M021769/1
• 负责人: Rob Field
• 金额: $ 26.03万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM021769%2F1
• 关键词: Glyconanoparticle; diagnostics; detection; discrimation; human

Recurring incidents of avian and swine flu infection in humans have prompted the development of novel, accurate point-of-care diagnostics for influenza. Current methods for the detection of influenza all take at least several hours and require specialist equipment and trained analysts. Rapid diagnostic tests do exist, but all are relatively crude and unable to distinguish between seasonal, pandemic and animal strains of influenza virus. The aim of this project is to develop a simple diagnostic test, based on practical and inexpensive gold nanoparticle-based colorimetric assays. Gold nanoparticles in aqueous solution exhibit an intense red colour that upon clustering of the nanoparticles changes colour to purple - readily observed with the naked eye. By functionalising gold nanoparticles with specific carbohydrate ligands, glyconanoparticles capable of clustering on flu virus surfaces will be developed, thus detecting and discriminating between human and animal strains.

人类感染禽流感和猪流感的事件不断发生，促使人们开发出新型、准确的流感即时诊断方法。目前检测流感的方法都至少需要几个小时，并且需要专业设备和训练有素的分析人员。快速诊断测试确实存在，但都相对粗糙，无法区分流感病毒的季节性、大流行和动物毒株。该项目的目的是开发一种简单的诊断测试，基于实用且廉价的金纳米颗粒比色测定。水溶液中的金纳米粒子呈现出强烈的红色，当纳米粒子聚集时，颜色会变成紫色——用肉眼很容易观察到。通过用特定的碳水化合物配体功能化金纳米颗粒，将开发出能够聚集在流感病毒表面的糖纳米颗粒，从而检测和区分人类和动物毒株。

项目成果

Detection of mSiglec-E, in solution and expressed on the surface of Chinese hamster ovary cells, using sialic acid functionalised gold nanoparticles.

使用唾液酸功能化金纳米颗粒检测溶液中并在中国仓鼠卵巢细胞表面表达的 mSiglec-E。

• DOI: 10.1039/c6an01230b
• 发表时间: 2016
• 期刊: The Analyst
• 作者: Schofield CL