TIMING OF MAMMALIAN CELL CYCLE EVENTS

哺乳动物细胞周期事件的发生时间

基本信息

批准号：
3193210
负责人：
ROBERT R KLEVECZ
金额：
$ 13.22万
依托单位：
BECKMAN RESEARCH INSTITUTE/CITY OF HOPE
依托单位国家：
美国
项目类别：
财政年份：
1989
资助国家：
美国
起止时间：
1989-04-10 至 1992-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3193210
关键词：
DNA replication abdomen neoplasm bone marrow breast neoplasms cell growth regulation cellular oncology circadian rhythms flow cytometry fluorescence microscopy gastrointestinal neoplasms human subject lavage therapy neoplasm /cancer chemotherapy neoplastic growth ovary neoplasms surgery tissue /organ preservation tumor antigens

项目摘要

The aim of this project is to reappraise the evidence for circadian cell kinetic changes in proliferation of human cancer cells in situ using modern methods, and to determine whether the proliferative activity of these tumor and nontumor cells changes in response to surgery or is perturbed by chemotherapy. Early attempts to make cell kinetic measurements in humans were limited by available methodology and, with few exceptions, ignored the circadian gating of cell proliferation. Tumor cells as well as normal tissues in patients with ovarian cancer show profound day to night variations in the percentage of cells in a phase that might be exploited for therapeutic benefit. Proliferation of tumor and nontumor diploid cells will be measured in patients with abdominal carcinomatosis prior to and following surgery and prior to and following chemotherapy using flow cytometry and fluorescence microscopy to measure DNA content, tumor specific antigen immunofluorescence, narrow angle and 90 degree scatter and BUdR immunofluorescence. Tumor cell proliferation maxima and kinetic indices will be compared with those taken from the bone marrow and the labeling and release of tumor cells from the solid tumor into the peritoneum will be measured.

该项目的目的是重新评估昼夜节律的证据人类癌细胞原位增殖的细胞动力学变化使用现代方法，并确定是否增殖这些肿瘤和非肿瘤细胞的活性发生变化以响应手术或受到化疗的干扰。早期对人类细胞动力学测量的尝试是受到可用方法的限制，除了少数例外，被忽略细胞增殖的昼夜节律门控。肿瘤细胞也一样卵巢癌患者的正常组织表现出深刻的一天某个阶段细胞百分比的夜间变化可能会被用于治疗效果。将测量肿瘤和非肿瘤二倍体细胞的增殖在术前和术后患有腹部癌的患者手术以及化疗前后使用流量细胞计数和荧光显微镜测量 DNA 含量、肿瘤特异性抗原免疫荧光，窄角和90度散射和 BUdR 免疫荧光。肿瘤细胞增殖最大值和动力学指数将为与从骨髓中提取的以及标记和将肿瘤细胞从实体瘤释放到腹膜中将被测量。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Automatic digital image analysis for identification of mitotic cells in synchronous mammalian cell cultures.

DOI：
发表时间：
1986-06
期刊：
Analytical and Quantitative Cytology and Histology
影响因子：
0
作者：
Eccles Ba;Klevecz Rr
通讯作者：
Eccles Ba;Klevecz Rr

Cellular clocks and oscillators.

DOI：
10.1016/s0074-7696(08)60178-3
发表时间：
1984
期刊：
International review of cytology
影响因子：
0
作者：
R. Klevecz;S. Kauffman;R. Shymko
通讯作者：
R. Klevecz;S. Kauffman;R. Shymko

Autogenous formation of spiral waves by coupled chaotic attractors.

通过耦合混沌吸引子自生形成螺旋波。

DOI：
10.3109/07420529109063914
发表时间：
1991
期刊：
Chronobiology international
影响因子：
2.8
作者：
Klevecz,RR;Pilliod,J;Bolen,J
通讯作者：
Bolen,J

Phase I trial of intraperitoneal iododeoxyuridine with and without intravenous high-dose folinic acid in the treatment of advanced malignancies primarily confined to the peritoneal cavity: flow cytometric and pharmacokinetic analysis.

腹腔内碘脱氧尿苷联合或不联合静脉注射大剂量亚叶酸治疗主要局限于腹膜腔的晚期恶性肿瘤的 I 期试验：流式细胞术和药代动力学分析。

DOI：
发表时间：
1998
期刊：
Cancer research.
影响因子：
0
作者：
MorganJr,RJ;Newman,EM;Doroshow,JH;McGonigle,K;Margolin,K;Raschko,J;Chow,W;Somlo,G;Leong,L;Tetef,M;Shibata,S;Hamasaki,V;Carroll,M;Vasilev,S;Akman,S;Coluzzi,P;Wagman,L;Longmate,J;Paz,B;Yen,Y;Klevecz,R
通讯作者：
Klevecz,R

Circadian and ultradian cytokinetic rhythms of spontaneous human cancer.

自发性人类癌症的昼夜节律和超节律细胞分裂节律。