ANTITUMOR AGENTS FROM HIGHER PLANTS

来自高等植物的抗肿瘤剂

• 批准号: 3193849
• 负责人: CHING-JER CHANG
• 金额: $ 13.77万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-07-01 至 1995-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3193849
• 关键词: X ray crystallography; antineoplastics; bioassay; chemical structure; clone cells; cytotoxicity; drug screening /evaluation; mass spectrometry; nuclear magnetic resonance spectroscopy; plant extracts

DESCRIPTION (Investigator's Abstract): This research project is aimed at the isolation and structural elucidation of novel antitumor agents from higher plants which have confirmed bioactivities. The prescreening bioassays are based on disease-oriented cytotoxicity against human tumor cell lines: A-549 (non-small cell lung cancer), HT-29 (colon cancer), and MCF-7 (breast cancer). Fractionation and purification of active constituents will be directed by selected bioassay. The structures of novel compounds will be elucidated by modern spectroscopic methods, including high field two-dimensional NMR, solid state NMR, mass spectrometry and X-ray crystallography. All active compounds will be submitted to the National Cancer Institute for further in vitro human tumor cell panels as well as in vivo xenograft evaluations. During the course of this investigation, continuing collection and prescreening of additional plants from Taiwan, North America, and India will be carried out to ensure reliable supplies of new active plants.

描述（研究者的摘要）：该研究项目的目的是 新型抗肿瘤剂的隔离和结构阐明 较高的植物证实了生物活性。 预筛选 生物测定基于面向疾病的细胞毒性对人肿瘤 细胞系：A-549（非小细胞肺癌），HT-29（结肠癌）和 MCF-7（乳腺癌）。主动分馏和纯化 成分将由选定的生物测定指导。 结构 新型化合物将通过现代光谱法阐明， 包括高场二维NMR，固态NMR，质量 光谱和X射线晶体学。 所有活性化合物将是 提交给国家癌症进一步的体外人类 肿瘤细胞板以及体内异种移植评估。 在 这项调查的过程，持续收集和预筛选 台湾，北美和印度的其他植物将被携带 以确保新的活跃植物的可靠供应。