ENHANCEMENT AND MODULATION OF ANTI-SENSE RNA ACTIVITY

反义 RNA 活性的增强和调节

基本信息

批准号：
3191379
负责人：
JONATHAN G IZANT
金额：
$ 10.15万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-04-01 至 1991-09-29
项目状态：
已结题

项目摘要

Antisense RNA transcribed from "flipped gene" plasmids is an effective method for cell autonomous suppression of exogenous (e.g. viral) as well as endogenous gene activity. We propose to expand our research program on anti-sense RNA to include two additional facets which will encompass RFA #87-CA-18 "Studies of Functional Anti-sense RNA in Oncogenic Viral Systems". Anti-sense gene chimeras will be constructed with URNA, tRNA, and rRNA genes. The level of transcription and the anti-sense inhibition activity of the hybrid genes will be tested in Xenopus oocytes and cultured mammalian cells. The goal of these studies is to exploit both the extremely active promoter signals in these structural RNA genes and, if possible, harness some of the special biological activity of the URNA, tRNA, and rRNA genes to enhance anti-sense RNA activity. Factors that modulate anti-sense RNA activity will be assessed in cultured mammalian cells and in Amphibian embryos. Somatic cell lines with high and low levels of RNA duplex destablizing activity will be selected and the nature of the "unwindase" characterized. Xenopus embryo germ layers and Xenopus tadpole tissues will be tested for unwindase activity that might contribute to tissue- specific gene expression. The capacity of the aforementioned chimeric anti-sense RNAs to overcome the unwindase block will be assessed in each system. Our goal is both to understand and to circumvent this crucial aspect of in vivo RNA-RNA interaction.

从“翻转基因”质粒转录的反义RNA是一种细胞自主抑制外源性（例如病毒）以及内源基因活性。我们建议扩大我们的反义RNA研究计划包括另外两个将涵盖 RFA #87-CA-18“功能研究”的各个方面致癌病毒系统中的反义RNA”。反义基因嵌合体将由URNA、tRNA和 rRNA基因。转录水平和反义水平杂交基因的抑制活性将在非洲爪蟾中进行测试卵母细胞和培养的哺乳动物细胞。这些研究的目标是利用这些中极其活跃的启动子信号结构RNA基因，如果可能的话，利用一些特殊的 URNA、tRNA 和 rRNA 基因的生物活性，以增强反义RNA活性。调节反义 RNA 活性的因素将在培养的哺乳动物细胞和两栖动物胚胎。体细胞具有高水平和低水平 RNA 双链体不稳定活性的品系将被选择并表征“unwindase”的性质。非洲爪蟾胚胎胚层和非洲爪蟾蝌蚪组织将被测试可能有助于组织的解旋酶活性特定的基因表达。上述容量克服解旋酶阻断的嵌合反义RNA将是在每个系统中进行评估。我们的目标是理解并规避体内 RNA-RNA 相互作用的这一关键方面。