REGULATION OF IA GENE EXPRESSION

IA基因表达的调控

• 批准号: 3177835
• 负责人: Jerold G Woodward
• 金额: $ 11.92万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3177835
• 关键词: clone cells; gene expression; genetic manipulation; genetic mapping; genetic regulation; immune response genes; immunoglobulin A; messenger RNA; tissue mosaicism

Our research will include studying the tissue distribution of murine Ia gene expression by analyzing the presence of Ia-specific messenger RNAs in tissues and cell lines. We will use DNA probes derived from four cloned Ia genes, A-alpha, A-beta, E-alpha, and E-beta, in combination with three sensitive RNA assays, dot blots, Northern blots, and S1 nuclease mapping, to critically evaluate the tissues capable of Ia gene expression as well as their capacity for Ia gene induction. S1 nuclease mapping will aIso be used to determine if there is any alternative processing of Ia mRNA. Our investigstion will determine the ability of nonbone marrow-derived cells to express Ia genes by producing radiation chimeras. The characteristics of Ia gene induction in both tissues and cell lines including macrophages, dendritic cells, and T cells will be evaluated using known inducing agents including gamma interferon. We intend to critically test a recent hypothesis that Ia antigens are induced in grafted tissue on cells other than "passenger leukocytes" as a consequence of the allograft reaction. Finally, we will study the mechanism of Ia gene induction by transfecting cloned Ia genes into different cell types and selecting for inducible expression. (MB)

我们的研究将包括研究鼠IA的组织分布 基因表达通过分析IA特异性信使RNA的存在 组织和细胞系。 我们将使用来自四个克隆IA的DNA探针 基因，α，a-beta，e-alpha和e-beta，结合三个 敏感的RNA分析，点印迹，北印迹和S 1核酸酶映射， 批判性评估能够IA基因表达的组织以及 它们的IA基因诱导能力。 S 1核酸酶映射将是AISO 用于确定IA mRNA是否有任何替代处理。 我们的 研究将确定非主骨髓衍生细胞的能力 通过产生辐射嵌合体来表达IA基因。 的特征 组织和细胞系中的IA基因诱导，包括巨噬细胞， 树突状细胞和T细胞将使用已知诱导剂进行评估 包括伽马干扰素。 我们打算批判性测试 假设Ia抗原是在细胞上移植组织中诱导的 由于同种异体移植反应，而不是“乘客白细胞”。 最后，我们将通过转染来研究IA基因诱导的机制 克隆的IA基因进入不同的细胞类型，并选择诱导 表达。 （MB）