TUMOR ANTIGEN CA125 IN OVARIAN CANCER PATIENTS

卵巢癌患者的肿瘤抗原 CA125

• 批准号: 3180280
• 负责人: TIMOTHY J O'BRIEN
• 金额: $ 10.61万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3180280
• 关键词: adenocarcinoma; antibody; carbohydrate structure; cell membrane; gel electrophoresis; gene expression; genetic library; growth /development; high performance liquid chromatography; human subject; laboratory mouse; laboratory rabbit; messenger RNA; molecular cloning; neoplasm /cancer genetics; neoplasm /cancer immunodiagnosis; neoplasm /cancer immunology; neoplasm /cancer relapse /recurrence; neoplasm /cancer therapy; neoplastic cell; nucleic acid hybridization; nucleic acid probes; oncoproteins; ovary neoplasms; patient care management; surface antigens; tumor antigens

CA125 is expressed on the surface of ovarian carcinoma cells. It is either sloughed or secreted so that significant levels are detected in serum. Serum levels of CA125 correlate with progression or regression of clinical disease and thereby provide a monitoring system for the therapeutic management of these patients. Neither the functional relationship of CA125 to malignancy (if any) or the structure of CA125 have been established. It has been established that CA125 is part of a mucin-like carbohydrate complex and that CA125 is intimately associated with the apocrine secretion process of the endometrium. Recently it has been possible to dissociate the CA125 complex into its component substructure which indicates that CA125 epitopes are present on a 66kD, 97kD and 115kD subunits. It is the objective of this proposal to understand the role (or co-incidental expression) of CA125 in malignancy as well as the biology of the CA125 molecule in normal growth and development. To do so, and to provide the basis for such an understanding it is proposed to structurally analyze the purified subunits of the CA125 molecule and to develop both antibodies and sequence information for use as reagents to clone and characterize the CA125 gene(s).

CA125在卵巢癌细胞表面表达。 它要么脱落，要么分泌，因此显着水平 血清中检测到。 CA125 的血清水平与 临床疾病的进展或消退，从而提供 用于这些疾病治疗管理的监测系统 患者。 CA125 与 恶性肿瘤（如果有）或 CA125 的结构已被确定 已确立的。 已确定 CA125 是 CA125 与粘蛋白样碳水化合物复合物密切相关 与子宫内膜的顶浆分泌过程有关。 最近，已经可以将 CA125 复合物解离成 它的子结构表明 CA125 表位是 存在于 66kD、97kD 和 115kD 亚基上。 这是目标 了解此提案的作用（或巧合 CA125 在恶性肿瘤中的表达）及其生物学 CA125分子正常生长发育。 为此，并 为这种理解提供基础 对 CA125 分子的纯化亚基进行结构分析 并开发抗体和序列信息以用作 用于克隆和表征 CA125 基因的试剂。