DYNAMICS OF CANCER CELL TRAFFIC IN THE LYMPHATIC SYSTEM

淋巴系统中癌细胞交通的动态

基本信息

批准号：
3176969
负责人：
LEONARD WEISS
金额：
$ 7.48万
依托单位：
NEW YORK STATE DEPARTMENT OF HEALTH
依托单位国家：
美国
项目类别：
财政年份：
1985
资助国家：
美国
起止时间：
1985-01-01 至 1988-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3176969
关键词：
adenocarcinoma autoradiography breast neoplasms capillary bed cell sorting circulating neoplastic cell fluorescent dye /probe lung neoplasms lymph nodes lymphatic circulation metastasis microinjections neoplasm /cancer classification /staging neoplasm /cancer diagnosis neoplasm /cancer transplantation radiotracer tissue /cell culture

项目摘要

Metastasis is of major importance in the diagnosis, staging, and treatment of cancer. In the case of carcinomas, initially the lymphatic route of dissemination is of particular importance, and in the later stages of disease, the hematogenous routes become progressively more important. Previous studies of the lung-to-liver and liver-to-lung traffic of cancer cells via the blood-stream in mice and rats, have demonstrated that the patterns of tumor growth are considerably modified by the interactions of cancer cells with the vascular beds of the first organ they encounter after gaining access to the blood-stream. However, analogous quantitative data on the traffic of cancer cells in the lymphatic system is lacking. It is proposed to obtain such dynamic data by introducing cancer cells into the lymphatic system of rats and mice via foot-pad injections; via transplanted subcutaneous tumors; and, in rats only, by direct intralymphatic injections at carefully controlled rates. By means of direct cell counts, bioassays, and the use of radiolabeled cancer cells, cancer cell traffic and viability will be quantitated in an orderly anatomic manner by sampling from the (prepopliteal) afferent lymph-vessels, the (popliteal) nodes, the (post-popliteal) efferent lymph-vessels, the next lymph-nodes "in-line", the venous blood by right ventricular puncture, and finally the lungs. Cancer cell traffic through lymph-nodes containing cancers of the same type will also be analyzed to differentially assess involved lymph-nodes as filters and/or generalizing sites. Although parts of the traffic of cancer cells with the lymphatic system have been described separately, there appears to have been no comprehensive, quantitative dynamic studies, as proposed here, on one of the major metastatic routes. This information is vital for any quantitation of the metastatic process. (L)

转移在诊断，分期和治疗中至关重要癌症。对于癌，最初是传播特别重要，在后来的阶段疾病，血源途径变得越来越重要。先前关于癌症肺到肝脏和肝脏流量的研究细胞通过小鼠和大鼠的血液流，已经证明了肿瘤生长的模式通过相互作用大大改变了癌细胞与他们遇到的第一个器官的血管床获得血液通道。但是，类似的定量数据缺乏淋巴系统中癌细胞的流量。这是提议通过将癌细胞引入到大鼠和小鼠通过足部注射的淋巴系统；通过移植皮下肿瘤；而且，仅在大鼠中，直接通过膜内注射以精心控制的速率。通过直接细胞计数，生物测定，以及使用放射标记的癌细胞，癌细胞流量和生存能力通过从（前）传入的淋巴管，（popliteal）节点，（后流行后）传出淋巴管，下一个淋巴结“在线”，右心穿刺的静脉血，最后是肺。癌细胞通过含有相同类型癌症的淋巴结的癌细胞流量还将分析以差异化评估涉及的淋巴结作为过滤器和/或概括地点。虽然癌细胞流量的一部分与淋巴系统已经单独描述了，似乎没有如这里提出的全面，定量的动态研究，主要的转移路线。此信息对于任何人都至关重要转移过程的定量。（L）