STUDIES ON EPITECTIN AND RELATED GLYCOPROTEINS

表观素及相关糖蛋白的研究

• 批准号: 3177106
• 负责人: VEER P. BHAVANANDAN
• 金额: $ 16.19万
• 依托单位: PENNSYLVANIA STATE UNIV HERSHEY MED CTR
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1994-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3177106
• 关键词: antibody formation; athymic mouse; carbohydrate sequence; complementary DNA; epitope mapping; glycoprotein biosynthesis; glycoprotein structure; glycoproteins; human tissue; laboratory mouse; laboratory rabbit; larynx neoplasms; melanoma; messenger RNA; molecular cloning; monoclonal antibody; mucins; neoplasm /cancer immunodiagnosis; neoplasm /cancer immunology; neoplastic cell culture for noncancer research; oligosaccharides; posttranslational modifications; protein structure; protein structure function; surface antigens; tissue /cell culture; tumor antigens; urinalysis

The cell surface has been one of the focal points in the studies on malignancy. General changes in structures of cell surface glycoconjugates as well as the presence of unique glycoconjugates in malignant cell surface have been reported. The long-range objective of this study is to contribute to the structure-function relationships of cancer cell surface glycoproteins and malignancy. The emphasis will be on the high molecular weight cell surface sialoglycoproteins (termed Ca antigen) which are detectable by Cal monoclonal antibody on a wide variety of human malignant cells. The primary objective is to carry out full biochemical characterization of the Ca antigen. Cal monoclonal antibody will be employed as affinity ligand for the isolation of the Ca antigen from human laryngeal carcinoma cells. The 390K and 350K species of the Ca antigen will be purified and their detailed chemical structure elucidated. The carbohydrate and protein composition, oligosaccharide type and location, and subunit structure are some of the aspects which will be studied. The counterpart of Ca antigen from human melanoma cells and the Ca antigen-like glycoproteins present in normal human urine will also be purified. The structural and immunological relationships of these materials and Ca antigen will be established. Polyclonal and monoclonal antibodies to the purified Ca antigen glycoproteins will be produced and used for understanding the above relationship. It is hoped that the information obtained in these studies will help to establish the potential use of Ca antigen as a human tumor marker. This glycoprotein antigen could be of use for the early diagnosis of cancer, for monitoring the course of disease, for localizing tumors, and for directing drugs to the tumor. (1)

细胞表面一直是研究的焦点之一 恶性。 细胞表面糖缀合物结构的一般变化 以及在恶性细胞表面存在独特的糖缀合物 已有报道。 这项研究的远程目标是 有助于癌细胞表面的结构功能关系 糖蛋白和恶性肿瘤。 重点将放在高分子上 体重细胞表面唾液糖蛋白（称为Ca抗原）是 通过Cal单克隆抗体可检测到各种各样的人类恶性肿瘤 细胞。 主要目的是进行完整的生化 Ca抗原的表征。 CAL单克隆抗体将是 用作亲和力配体从人类分离Ca抗原 喉癌细胞。 CA抗原的390K和350K种 将被纯化，并阐明其详细的化学结构。 这 碳水化合物和蛋白质成分，寡糖类型和位置， 亚基结构是将要研究的一些方面。 这 来自人类黑色素瘤细胞和ca抗原样的Ca抗原的对应物 正常人尿液中存在的糖蛋白也将被纯化。 这 这些材料和CA的结构和免疫关系 将建立抗原。 多克隆和单克隆抗体 将生产纯化的Ca抗原糖蛋白并用于 了解上述关系。 希望这些信息 在这些研究中获得的将有助于确定CA的潜在用途 抗原作为人类肿瘤标记。 这种糖蛋白抗原可能是使用的 为了早期诊断癌症，以监测疾病的病程 用于定位肿瘤，并将药物引导到肿瘤。 （1）