QUANTITATIVE AUTORADIOGRAPHY OF RADIATED BRAIN TUMOR

放射脑肿瘤的定量放射自显影

• 批准号: 3171731
• 负责人: ALEXANDER M SPENCE
• 金额: $ 10.82万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-02-01 至 1987-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3171731
• 关键词: astrocytoma; autoradiography; blood brain barrier; brain circulation; brain neoplasms; deoxyglucose; disease /disorder model; gamma radiation; glucose metabolism; mathematical model; membrane permeability; neoplasm /cancer radionuclide therapy; radiotracer

This program has two interrelated objectives. The first is to validate and extend current quantitative autoradiographic methods (QAR) for measurement of blood flow, vascular permeability, and regional glucose metabolism to brain tumors and irradiate brain. The second objective is to develop a detailed understanding of the time course of the irradiation response of brain tumor, (and where possible, normal brain) with respect to blood flow, permeability, and glucose metabolism. The research plan consists of five main experiments to be performed on normal rat brain and intracerebrally-transplanted rat malignant astrocytic gliomas under conditions of treatment with fractionated and unfractionated Cs-137 gamma irradiation. Blood flow will be examined by means of radiolabelled microsphere methods and QAR with the tracer, C-14 iodoantipyrine. Capillary permeability measurements will use QAR with C-14 Alpha-aminoisobutyric acid. Assessment of regional glucose metabolism will be based on current kinetic models which use C-14 deoxyglucose. A principal part of this work will be (a) to validate and refine some of the current QAR methods for measuring blood flow, permeability, and glucose metabolism, and (b) to explore new mathematical models that may more accurately portray these facets of brain tumor biology. This study will provide pathophysiologic information about brain tumor radiation response which will be useful in planning other treatment interventions, such as the timing of adjunctive chemotherapy, and treatment with radioprotectors, or radiosensitizers. Also, this project will serve as a scientific basis for the transfer of QAR-based measurement techniques from laboratory animals with brain tumors to human patients with malignant brain tumors studied with positron emission tomography.

该程序具有两个相互关联的目标。 首先是验证和 扩展电流定量放射自显影方法（QAR）进行测量 血流，血管渗透性和区域葡萄糖代谢 脑肿瘤和照射大脑。 第二个目标是开发 详细了解辐射响应的时间过程 关于血流 渗透性和葡萄糖代谢。 研究计划包括五个 在正常大鼠大脑上进行的主要实验， 脑部移植的大鼠恶性星形细胞神经胶质瘤 用分离和未分离的CS-137伽马治疗条件 辐照。 血流将通过放射性标记检查 微球方法和带有示踪剂C-14 iodoantipyrine的QAR。 毛细管渗透率测量将与C-14一起使用QAR α-氨基异丁酸。 区域葡萄糖代谢的评估将 基于使用C-14脱氧葡萄糖的当前动力学模型。 一个 这项工作的主要部分将是（a）验证和完善一些 当前测量血流，渗透性和葡萄糖的QAR方法 代谢，（b）探索可能更多的新数学模型 准确地描绘了脑肿瘤生物学的这些方面。 这项研究会 提供有关脑肿瘤辐射反应的病理生理信息 这对于计划其他治疗干预措施，例如 辅助化疗的时间，以及用辐射保护剂治疗或 放射增敏剂。 此外，该项目将作为科学依据 基于QAR的测量技术从实验动物转移 研究了脑肿瘤的恶性脑肿瘤患者 与正电子发射断层扫描。