DIETARY MINERALS AND FATS IN BREAST CANCER

膳食矿物质和脂肪与乳腺癌的关系

• 批准号: 3167779
• 负责人: CLEMENT C IP
• 金额: $ 12.07万
• 依托单位: ROSWELL PARK CANCER INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-04-01 至 1986-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3167779
• 关键词: benzanthracenes; blood tests; breast neoplasms; butylated hydroxytoluene; carcinogenesis inhibitor; cellular oncology; chemical carcinogenesis; diet therapy; dietary lipid; dietary supplements; dietary trace element; fluorimetry; glutathione peroxidase; methionine; nutrition related tag; peroxidation; saturated fat; sectioning; selenium; stainings; tissue /cell preparation; tocopherols; unsaturated fats; vegetable oils

It is the objective of this proposal to continue studies on the anticarcinogenic action of selenium in mammary neoplasia and to determine how the feeding of a high-fat diet can alter the chemopreventive efficacy of selenium. Our investigation involves the dimethylbenz(a)anthracene (DMBA)-induced mammary tumor model in female SD rats. Aim 1 is designed to compare different selenium compounds, both organic and inorganic, with respect to their effectiveness as chemopreventive agents. Furthermore, we are interested in finding out if the prophylactic potency of different selenium compounds is related to the levels of selenium in the mammary gland and blood. Aim 2 is designed to study the synergistic action of selenium and vitamin E in the inhibition of mammary tumorigenesis. This project is a follow-up of our previous observation that vitamin E is able to potentiate the anticarcinogenic action of selenium. First, we plan to find out if lower levels of vitamin E can be used without compromising its effectiveness, and secondly, to determine if the combination of selenium and vitamin E supplementation can obliterate the differential effect of high-fat and low-fat diets on mammary carcinogenesis. Finally, the effects of selenium/vitamin E supplementation on certain aspects of the host defense system as influenced by fat intake will be evaluated. Aim 3 is designed to delineate if the action of selenium is unique and distinct from other synthetic phenolic antioxidants such as butylated hydroxyanisole (BHA). The effects of selenium will be compared to some of the well known effects of BHA, specifically the selective induction of various liver enzymes that are responsible for the detoxification and inactivation of xenobiotics. Aim 4 is designed to determine if selenium stimulates the DNA repair mechanism after carcinogenic injury. We have previously demonstrated that suppression of mammary tumorigenesis was observed when selenium supplementation was limited to the time of carcinogen administration. We therefore plan to examine the effect of selenium on the binding of DMBA to mammary cell DNA and on the excision repair process as measured by unscheduled DNA synthesis. It cannot be overemphasized that a better understanding of the diverse action of selenium is essential before its usefulness in chemoprevention can be properly evaluated.

该提议的目的是继续研究 硒在乳腺肿瘤中的抗癌作用并确定 高脂饮食的喂养如何改变化学预防功效 硒。 我们的调查涉及二甲基苯甲酸（a）蒽 （DMBA）在雌性SD大鼠中诱导的乳腺肿瘤模型。 AIM 1旨在 将不同的硒化合物（有机和无机化合物）与 尊重它们作为化学预防剂的有效性。 此外，我们 有兴趣找出不同的预防效力 硒化合物与乳腺中的硒水平有关 腺体和血液。 AIM 2旨在研究 硒和维生素E在抑制乳腺肿瘤发生中。 这 项目是我们以前观察到维生素E的后续行动 增强硒的抗癌作用。 首先，我们计划 找出是否可以使用较低水平的维生素E 有效性，其次是确定硒的组合是否 维生素E补充可以消除 乳腺癌发生的高脂饮食和低脂饮食。 最后，效果 在宿主的某些方面补充硒/维生素E 将评估受脂肪摄入影响的防御系统。 AIM 3是 如果硒的作用是独特的，并且不同于 其他合成酚类抗氧化剂，例如丁基化的羟基烷溶剂 （bha）。 硒的影响将与一些已知的 BHA的影响，特别是各种肝的选择性诱导 负责排毒和失活的酶 异种生物。 AIM 4旨在确定硒是否刺激DNA 致癌损伤后修复机制。 我们以前有 证明当乳腺肿瘤发生抑制时 补充硒是致癌时间的 行政。 因此，我们计划检查硒对 DMBA与乳细胞DNA和切除修复过程的结合为 通过未定义的DNA合成测量。 不能过分强调 更好地理解硒的多种作用是必不可少的 可以正确评估其在化学预防中的有用性。