TRANSFECTION BY ENDOGENOUS HUMAN TRANSFORMING GENES

内源性人类转化基因的转染

• 批准号: 3168442
• 负责人: GEOFFREY M COOPER
• 金额: $ 16.63万
• 依托单位: DANA-FARBER CANCER INST
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-05-01 至 1989-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3168442
• 关键词: B lymphocyte; Burkitt's lymphoma; DNA; antiserum; embryo /fetus tissue /cell culture; gene expression; genetic manipulation; genetic mapping; genetic recombination; human tissue; lymphoma; molecular cloning; multiple myeloma; neoplasm /cancer genetics; neoplastic cell; neoplastic transformation; nucleic acid sequence; radiotracer; tissue /cell culture

The overall goal of the research is the analysis of cellular-transforming genes in human neoplasms. We will focus on the nonvirus-related transforming genes that are activated in human B-lymphocyte neoplasms and detected by transfection of NIH 3T3 cells. These genes will be characterized by molecular cloning and nucleotide sequencing in order to identify their gene products and to determine the molecular alterations responsible for activation of their transforming potential in neoplastic cells. Antisera against predicted gene products will be used for characterization of these transforming proteins. The role of these transforming genes in lymphomagenesis will be investigated by transfection of human B lymphocytes. (X)

该研究的总体目标是分析细胞转化 人类肿瘤中的基因。 我们将重点关注与病毒无关的 转化在人类 B 淋巴细胞肿瘤中激活的基因 通过转染 NIH 3T3 细胞来检测。 这些基因将 通过分子克隆和核苷酸测序来表征 鉴定其基因产物并确定分子改变 负责激活其在肿瘤中的转化潜力 细胞。 针对预测基因产物的抗血清将用于 这些转化蛋白的表征。 这些的作用 将通过转染研究淋巴瘤发生中的转化基因 人类 B 淋巴细胞。 （十）