INTERNEURONAL MICROCIRCUITRY OF THE RAT NEOSTRIATUM

大鼠新纹状体的神经元微循环

• 批准号: 2891128
• 负责人: James M Tepper
• 金额: $ 7.58万
• 依托单位: RUTGERS THE STATE UNIV OF NJ NEWARK
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2891128
• 关键词: action potentials; biocytin; brain electrical activity; calcium binding protein; cell population study; corpus striatum; electrical potential; electron microscopy; gamma aminobutyrate; immunocytochemistry; interneurons; laboratory rat; light microscopy; neural information processing; somatostatin; stainings; voltage /patch clamp

DESCRIPTION (Adapted from applicant's abstract): The basal ganglia are critically involved in the organization of motor behavior and sensorimotor integration. Malfunctions of this system in humans contribute to the pathophysiology of neuropsychiatric disorders such as schizophrenia and obsessive compulsive disorder, as well as to neurological diseases such as Huntington's and Parkinson's disease. Understanding the information processing within this system in mechanistic terms requires the understanding of the physiological properties and anatomical organization of the microcircuitry of its constituent nuclei. The neostriatum, the largest nucleus of the basal ganglia has a central role in controlling the functioning of the basal ganglia and therefore, an understanding of the intrinsic operations of this nucleus is critical to understanding information processing in the basal ganglia. Recently, it has been increasingly recognized that the quantitatively minor population of GABAergic interneurons of the neostriatum may play a critical role in the organization of the population activity of the principal cells. However, until the recent introduction of visually guided whole cell recording these neurons were not accessible for physiological investigation. In the proposed study, this powerful technology will be used in combination with intracellular staining and light and electron microscopic analysis to analyze the role of these interneurons in the control of the activity of their principal postsynaptic targets, the medium spiny neuron. Simultaneous paired recordings will be obtained from interneurons and medium spiny cells and the neurons will be stained for further anatomical investigation. The following specific questions will be addressed. First, what is the nature of the synaptic interaction between the two major types of inhibitory inteneurons and the medium spiny neuron? Second, what are the physiological and anatomical specializations of the inhibitory inputs to the medium spiny neuron from different types of interneurons? Third, what is the pattern of connectivity among the populations of interneurons and medium spiny neurons (i.e. convergence and divergence)? Finally, what are the physiological properties of electrical coupling between interneurons, a potentially significant mechanism affecting the population activity of these cells, as well as, the medium spiny neurons? This data will help in understanding the contribution of these interneurons to the organization of activity in the neostriatum.

描述（改编自申请人的摘要）：基底神经节是 关键参与运动行为和感觉运动的组织 一体化。 人类这个系统的故障会导致 神经精神疾病的病理生理学，例如精神分裂症和 强迫症，以及神经系统疾病，例如 亨廷顿氏病和帕金森氏病。 了解信息 从机械角度来说，该系统内的处理需要 了解生理特性和解剖组织 其组成核的微电路。 新纹状体是最大的 基底神经节核在控制 基底神经节的功能，因此，了解 该核的内在运作对于理解至关重要 基底神经节的信息处理。 最近，已经 人们越来越认识到，人口数量较少 新纹状体的 GABA 能中间神经元可能在 主细胞群体活动的组织。 然而， 直到最近引入视觉引导的全细胞记录这些 神经元无法进行生理研究。 在 拟议的研究，这项强大的技术将与 细胞内染色以及光镜和电子显微镜分析 分析这些中间神经元在控制活动中的作用 它们的主要突触后目标是中棘神经元。 同时 将从中间神经元和中型多刺细胞获得配对记录 神经元将被染色以进行进一步的解剖学研究。 这 将解决以下具体问题。 一、什么是本质 两种主要抑制类型之间的突触相互作用 中间神经元和中棘神经元？ 二、生理方面有哪些 以及中等刺的抑制性输入的解剖学特化 来自不同类型中间神经元的神经元？ 三、什么是格局 中间神经元和中等棘神经元群体之间的连接 （即收敛和发散）？ 最后，生理方面有哪些 中间神经元之间的电耦合特性，潜在的 影响这些细胞群体活动的重要机制，如 还有，中型多刺神经元？ 这些数据将有助于理解 这些中间神经元对组织活动的贡献 新纹状体。