Bilateral BBSRC-FAPESP - New approaches towards improved functionality of saccharolytic enzymes from fungi

双边 BBSRC-FAPESP - 改善真菌糖分解酶功能的新方法

• 批准号: BB/K01434X/1
• 负责人: David Archer
• 金额: $ 46.14万
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FK01434X%2F1
• 关键词: Bilateral; BBSRC; FAPESP; New; approaches

This project proposal brings together scientists from the UK and Brazil in a collaborative project, relating to the production of biofuels, where they each bring expertise. There is a need to find realistic alternatives to the use of fossil fuels worldwide and there are targets for the amount of energy to be provided from renewables in many countries including the UK and Brazil. Those targets are challenging but the production of biofuels from agricultural wastes is targeted to make a contribution to the energy needs of many countries. Brazil leads the world in the production of ethanol from the fermentation of sugar by yeast to ethanol ('bioethanol'). That is an example of a first-generation process but that is not realistic in the UK where crops are grown for food and there is no capacity to grow crops specifically for biofuels. Therefore, second generation biofuels are designed to use non-food crops and one way of achieving that is to use agricultural waste materials. Those wastes do not contain easily-fermented sugars but they do contain a lot of plant cell wall material that is comprised mainly of cellulose and other polymers. In the production of second generation biofuels, these polymers are first degraded by enzymes to sugars and the sugars are then converted to ethanol by yeast. That enzyme step uses enzymes from fungi (moulds) that do that job naturally. That natural capacity of moulds needs to be understood so that it can then be transferred to a successful industrial process. Our previous research has shown that different fungi approach the problem of degrading polymers in different ways and this project aims to learn from those fungi in order to optimise the process.

该项目提案将来自英国和巴西的科学家汇集在一个合作项目中，这与生产生物燃料的生产有关，他们每个人都带来了专业知识。有必要找到在全球使用化石燃料的现实替代品，并且有一些目标是在包括英国和巴西在内的许多国家提供可再生能源的能源。这些目标具有挑战性，但农业废物产生的生物燃料旨在为许多国家的能源需求做出贡献。巴西领导着世界，从酵母发酵糖的发酵到乙醇（“生物乙醇”）中。这是第一代过程的一个例子，但是在英国，农作物是为食物种植的，并且没有能力为生物燃料种植作物的能力。因此，第二代生物燃料旨在使用非食品作物和一种实现农业废料的方法。这些废物不含易于发酵的糖，但它们确实包含许多主要由纤维素和其他聚合物组成的植物细胞壁材料。在第二代生物燃料的生产中，这些聚合物首先被酶降解为糖，然后通过酵母将糖转化为乙醇。该酶步骤使用了自然完成该工作的真菌（模具）的酶。需要了解霉菌的自然能力，以便将其转移到成功的工业过程中。我们以前的研究表明，不同的真菌以不同方式降低聚合物的问题，该项目旨在向这些真菌学习以优化过程。

项目成果

Transcriptomic responses of mixed cultures of ascomycete fungi to lignocellulose using dual RNA-seq reveal inter-species antagonism and limited beneficial effects on CAZyme expression.

• DOI: 10.1016/j.fgb.2016.04.005
• 发表时间: 2017-05
• 期刊: Fungal genetics and biology : FG & B
• 作者: Daly P;van Munster JM;Kokolski M;Sang F;Blythe MJ;Malla S;Velasco de Castro Oliveira J;Goldman GH;Archer DB
• 通讯作者: Archer DB

MOESM11 of Expression of Aspergillus niger CAZymes is determined by compositional changes in wheat straw generated by hydrothermal or ionic liquid pretreatments

黑曲霉 CAZymes 表达的 MOESM11 由水热或离子液体预处理产生的麦秆的成分变化决定

• DOI: 10.6084/m9.figshare.c.3685393_d4
• 发表时间: 2017
• 作者: Daly P

Additional file 8 of Succession of physiological stages hallmarks the transcriptomic response of the fungus Aspergillus niger to lignocellulose

生理阶段演替的附加文件 8 标志着真菌黑曲霉对木质纤维素的转录组反应

• DOI: 10.6084/m9.figshare.12121851
• 发表时间: 2020
• 作者: Munster J

A solid state fungal fermentation-based strategy for the hydrolysis of wheat straw.

• DOI: 10.1016/j.biortech.2013.09.061
• 发表时间: 2013-12
• 期刊: Bioresource technology
• 影响因子: 11.4
• 作者: Pensupa N;Jin M;Kokolski M;Archer DB;Du C
• 通讯作者: Du C

Additional file 5 of Succession of physiological stages hallmarks the transcriptomic response of the fungus Aspergillus niger to lignocellulose

生理阶段演替的附加文件 5 标志着真菌黑曲霉对木质纤维素的转录组反应

• DOI: 10.6084/m9.figshare.12121842
• 发表时间: 2020
• 作者: Munster J