RNA DIRECTED DNA POLYMERASE & 70S RNA OF ONCORNAVIRUSES

RNA定向DNA聚合酶

基本信息

批准号：
2086700
负责人：
ANTHONY J FARAS
金额：
$ 12.45万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
1980
资助国家：
美国
起止时间：
1980-06-01 至 1994-11-30
项目状态：
已结题

项目摘要

The studies proposed in this renewal grant application exemplify a continuation of our ongoing work on the nature of retrovirus- related structures present in cell types previously purported to lack endogenous retroviruses. We have recently demonstrated that several species of avian cells previously thought to lack avian retrovirus-related sequences in fact contain such sequences in the form of two genetic structures. The structure of one group of viral-related genetic sequences exists as endogenous retroviruses, since they contain gag-, pol-, env and LTR-related sequences linked to one another in the form of a provirus. The structure of the other group of retrovirus-related genetic sequences exists as solo gag-, pol-, env-, and LTR-related sequences not linked to one another in the form of a provisus. In an effort to shed light on the nature of these novel families of avian endogenous retroviruses and the solo retrovirus-related genetic sequences present cell types, we plan to: 1) isolate and characterize both classes of avian retrovirus genetic sequences present in these cell types in order to delineate their precise nature; 2) identify and characterize the transcriptional related structures in other avian species as well as other vertebrate genera in an attempt to define the nature of more distantly-evolved sequences and novel classes of endogenous retroviruses. The methodologies that we plan to employ to achieve these objectives are, for the most part, already implemented in our laboratory and include a variety of physicochemical, enzymological, and hybridization techniques available for the analysis of DNA, RNA, and protein,

本更新授予申请中提出的研究举例说明我们正在进行的关于逆转录病毒本质的工作的延续以前称为细胞类型中存在的相关结构缺乏内源性逆转录病毒。我们最近证明了几种以前认为缺乏禽类的鸟类细胞实际上，与逆转录病毒相关的序列在两个遗传结构的形式。一组的结构病毒相关的遗传序列作为内源性逆转录病毒存在，由于它们包含链接的gag-，pol-，env和与LTR相关的序列彼此以病毒的形式彼此。结构其他与逆转录病毒相关的遗传序列作为独奏存在 gag-，pol-，env-和ltr相关序列与一个序列没有链接另一种是条约的形式。为了阐明这些新型禽内源性逆转录病毒的本质和独奏逆转录病毒相关的遗传序列存在细胞类型，我们计划：1）隔离和表征两类这些细胞类型中存在的禽逆转录病毒遗传序列为了描绘其精确的本质； 2）识别和表征其他鸟类的转录相关结构物种以及其他脊椎动物属，试图定义更遥远的序列和新颖类的本质内源性逆转录病毒。我们计划的方法实现这些目标的雇用大多数已经是在我们的实验室中实施，包括各种各样的物理化学，酶学和杂交技术可用于分析DNA，RNA和蛋白质，