METABOLISM OF NC-METHYLARGININES AND NEOPLASIA

NC-甲基精氨酸的代谢与肿瘤

• 批准号: 3163621
• 负责人: WOON K PAIK
• 金额: $ 9.29万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-06-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3163621
• 关键词: aminoacid metabolism; arginine; bacteria; carboxylation; cell differentiation; chemical carcinogen; chemical synthesis; chromatography; hepatocellular carcinoma; kidney; liver; liver regeneration; methylation; neoplastic cell; neoplastic growth; nitrosourea; radiotracer; tissue /cell culture; urea cycle

The most important objective in this research is to study the metabolism of NG-monomethyl-L-arginine, an endogenously synthesized compound, and to identify one of the metabolites as N-methylurea, a compound which can be nitrosated to give rise to 1-N-methyl,1-N-nitrosourea, one of the most potent carcinogens known. This will be pursued with the following aims: (1)\to study the metabolic pathways of NG-monomethyl-L-arginine which involve hydrolytic cleavage, oxidative demethylation, and decarboxylation; (2)\to investigate the possible formation of N-methylurea from NG-monomethyl-L-arginine; (3)\to further purify protein methylase I which methylates the guanadine group of protein-bound arginine residues; and (4)\to investigate the possible antitumor effects of NG-monomethyl-L-arginine on tumor cells in tissue or cell culture. (K)

本研究最重要的目的是研究代谢 NG-单甲基-L-精氨酸，一种内源合成的化合物，并且 确定其中一种代谢物为 N-甲基脲，这是一种可以被 亚硝化生成 1-N-甲基,1-N-亚硝基脲，是最常见的化学物质之一 已知强效致癌物。 我们将实现以下目标： (1)\研究NG-单甲基-L-精氨酸的代谢途径 涉及水解裂解、氧化脱甲基化和脱羧基化； (2)\研究N-甲基脲的可能形成 NG-单甲基-L-精氨酸； (3)\进一步纯化蛋白甲基化酶I， 甲基化蛋白质结合的精氨酸残基的胍基团；和 (4)\研究可能的抗肿瘤作用 NG-单甲基-L-精氨酸对组织或细胞培养物中肿瘤细胞的作用。 (K)