CHARACTERIZATION OF SUB-EPITHELIAL MATRIX ANTIGENS

上皮下基质抗原的表征

• 批准号: 3157342
• 负责人: ROBERT E BURGESON
• 金额: $ 5.71万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-23 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3157342
• 关键词: antibody; basement membrane; electron microscopy; enzyme linked immunosorbent assay; epidermolysis bullosa; epithelium; extracellular matrix; fibroblasts; gel electrophoresis; human tissue; immunofluorescence technique; monoclonal antibody; skin; skin disorder; surface antigens; tissue /cell culture

The basement membrane zone underlying the squamous epithelium of external tissues is ultrastructurally more complex than basement membrane zones of other tissues. In addition to lamina densa, these regions contain additional cellular elements such as the hemidesmosomes of the basal cells, anchoring filaments between the hemidesmosomes and the lamina densa, anchoring fibrils which appear to connect the lamina densa with the underlying connective tissue, and oxytalan fibers which may mediate the interaction of elastic elements with the lamina densa. The diseases of bullous pemphigoid and epidermolysis bullosa affect specific components in this region, and demonstrate the uniqueness of these structures to external tissues. As a first attempt to describe the network of molecules involved in the interaction of epithelial cells with molecules deep in the underlying matrix, we have produced monoclonal antibodies to yet unrecognized components. Two of these antibodies have been selected for further characterization. One of them have immunological and biochemical characteristics similar to those expected for anchoring filaments. The other has characteristics resembling oxytalan fibers. We have shown that these antigens are synthesized by specific cell populations and have demonstrated the feasibility of their chemical characterization. We propose to isolate and characterize these antigens in detail, to identify the antigen with ultrastructurally observed fibrous components of the basement membrane zone, and to investigate potential interactions between these components and other known connective tissue elements. We anticipate that the proposed studies will provide specific information regarding these basement membrane zone proteins and will help form a basis for the understanding of the structure of this unique region. This information will provide the background for forming hypotheses regarding the molecular events underlying the pathogenesis of epidermolysis bullosa and bullous pemphigoid.

外部鳞状上皮的基底膜区 组织比地下膜区更为复杂 其他组织。 除lamina densa外，这些区域还包含 其他细胞元素，例如基底细胞的半体体大体， 锚固符号在Hemidesmosomes和Lamina Densa之间， 锚定似乎将Lamina Densa与 潜在的结缔组织和氧气纤维可能介导 弹性元素与lamina densa的相互作用。 这些疾病 大胆的芽孢杆菌和表皮溶解Bulosa会影响特定的组成部分 该区域，并证明了这些结构对外部的独特性 组织。 作为描述参与的分子网络的首次尝试 上皮细胞与深处深处的分子的相互作用 矩阵，我们已经产生了单克隆抗体以但未识别 成分。 这些抗体中的两种已被选择进一步 表征。 其中一个具有免疫学和生化 特征类似于预期的锚定细丝的特征。 这 其他具有类似氧纤维的特征。 我们已经表明 这些抗原是由特定细胞群合成的，具有 证明了其化学表征的可行性。 我们 提议详细分离和表征这些抗原，以识别 具有超微结结构观察到的抗原的纤维成分 地下室膜区，并研究 这些成分和其他已知的结缔组织元素。 我们预计拟议的研究将提供特定的信息 关于这些地下室膜区域蛋白质，并将有助于形成基础 为了理解这个独特区域的结构。 这 信息将为形成有关假设的背景 表皮溶液发病机理的基础的分子事件 和大胆的幽灵。