SUGAR AND GLYCOPROTEIN DIGESTION IN CYSTIC FIBROSIS

囊性纤维化中的糖和糖蛋白消化

基本信息

批准号：
3153665
负责人：
Jay A Perman
金额：
$ 12.95万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-06-01 至 1987-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3153665
关键词：
breath tests carbohydrate metabolism cystic fibrosis dietary carbohydrates digestion disaccharides drug administration rate /duration enteric bacteria gastrointestinal nutrient absorption glucose human tissue malabsorption mucins noninvasive diagnosis orphan disease /drug small intestines

项目摘要

The overall objectives of the proposed research are 1. to quantify carbohydrate absorption in cystic fibrosis (CF); 2. to determine the effect of chronic oral antibiotic therapy on intestinal bacterial metabolism of malabsorbed dietary and endogenous carbohydrates and carbohydrates in glycoproteins; 3. to determine whether small bowel bacterial overgrowth is present in CF. The proposed approach takes advantage of the noninvasive, highly specific and sensitive techniques of breath analysis as well as in vitro fecal incubations previously shown to model in vivo colonic bacterial metabolism of carbohydrates. Excretion of H2 in breath will indicate the quantity of carbohydrate escaping small bowel absorption. The results obtained in vivo using breath measurements will be correlated with data in vitro obtained using measurements of bacterial metabolism of test substrates. Correlation of expired air and in vitro measurements will indicate any therapy-induced shifts in fermentation by the enteric bacterial ecosystem. Thus, variables affecting H2 production in vivo which are independent of absorptive capacity for a given sugar will be recognized. In addition, the fecal incubation system will permit investigation of intestinal bacterial metabolism of non-dietary endogenous glycoprotein substrates in CF. The carbohydrate portion of nutrient intake is of special importance in provision of energy to the CF patient, since loss of calories in the form of other macronutrients, principally fat, may be extensive. The present proposal should provide quantitative information acquired noninvasively on digestion and absorption of sugars in individuals with CF, and the data obtained should be directly applicable to dietary and pharmacologic management in these patients. Similarly, the proposed studies utilizing endogenous glycoproteins may provide important insights into the role of enteric bacteria in degradation of mucins accumulating in CF intestine.

拟议研究的总体目标是 1. 量化囊性纤维化 (CF) 中碳水化合物的吸收； 2. 判断效果慢性口服抗生素治疗对肠道细菌代谢的影响吸收不良的饮食和内源性碳水化合物以及碳水化合物糖蛋白； 3.确定小肠细菌是否过度生长存在于CF中。所提出的方法利用了非侵入性、高度特异性和灵敏的呼吸分析技术以及先前显示体外粪便培养可模拟体内结肠细菌碳水化合物的代谢。呼吸中 H2 的排泄将表明逃逸小肠吸收的碳水化合物的量。结果使用呼吸测量在体内获得的数据将与使用测试的细菌代谢测量体外获得基材。呼出气体与体外测量值的相关性将表明任何治疗引起的肠道发酵变化细菌生态系统。因此，影响体内 H2 产生的变量与给定糖的吸收能力无关认可。此外，粪便孵化系统将允许非膳食内源性肠道细菌代谢研究 CF 中的糖蛋白底物。营养素摄入中的碳水化合物部分在以下方面特别重要：为 CF 患者提供能量，因为卡路里的损失形式为其他常量营养素（主要是脂肪）的含量可能很广泛。现在的提案应提供非侵入性获得的定量信息 CF 患者糖的消化和吸收，以及数据获得的结果应直接适用于饮食和药理学对这些患者的管理。同样，拟议的研究利用内源性糖蛋白可能为了解内源性糖蛋白的作用提供重要的见解肠道细菌降解 CF 肠道中积聚的粘蛋白。