THE ACTION OF CALCIUM ON ADIPOSE TISSUE

钙对脂肪组织的作用

• 批准号: 3151372
• 负责人: RICHARD J SCHIMMEL
• 金额: $ 8.29万
• 依托单位: UNIV OF MED/DENT NJ-SCH OSTEOPATHIC MED
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-08-15 至 1988-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3151372
• 关键词: acclimatization; adenylate cyclase; adipocytes; adrenergic agents; alpha antiadrenergic agent; brown fat; calcium metabolism; catecholamines; cell membrane; cyclic AMP; environmental stressor; glucose metabolism; glycogen synthase; hormone regulation /control mechanism; ionophores; isoproterenol; lipolysis; membrane lipids; methoxamine; phentolamine; phenylephrine; phosphatidate; phospholipase C; phospholipids; phosphorylases; prazosin; radiotracer; respiratory pharmacology; tissue /cell culture; yohimbine

The overall objective of the proposed research is to evaluate the possible participation of calcium ion as an itnracellular messenger mediating hormone action on adipose tissue cells. Earlier studies using fat cells and other cell systems have provided data suggesting an action of calcium prior to and also antecedent to formation of cyclic AMP. In my laboratory, experiments to localize the site of calcium action on isolated fat cells are conducted by measuring cyclic AMP levels, rates of lipolysis and activity of the cyclic AMP dependent protein kinase in hamster adipocytes exposed to various stimuli and inhibitors of lipolysis. Subsequent studies will employ various pharmacologic agents which either promote (A23187 and X537A) or retard (verapamil and D600) calcium entry into cells. During the forthcoming year we shall continue our studies on hamster fat cells. Adipocytes from hamsters respond to alpha adrenergic agents with a partial inhibition of lipolysis and of cyclic AMP accumulation. We shall be looking at the interrelationships between calcium ion and alpha adrenergic control of lipolysis and cyclic AMP levels in fat cells from this species.

拟议研究的总体目的是评估可能 钙离子作为iTnracellular Messenger介导激素的参与 对脂肪组织细胞的作用。 使用脂肪细胞和其他细胞的早期研究 系统提供了建议在之前的钙作用的数据以及 循环AMP形成的先例。 在我的实验室中，实验 将钙作用部位定位在孤立的脂肪细胞上 测量环状AMP水平，脂解的速率和循环AMP的活性 暴露于各种刺激的仓鼠脂肪细胞中的依赖性蛋白激酶 脂解抑制剂。 随后的研究将采用各种药理学 促进（A23187和X537A）或延迟（Verapamil和D600）的代理 钙进入细胞。 在即将到来的一年中，我们将继续我们的 仓鼠脂肪细胞的研究。 仓鼠的脂肪细胞对α 肾上腺素能部分抑制脂解和环状AMP 积累。 我们将研究钙离子之间的相互关系 脂肪分解和循环AMP水平的α肾上腺素能控制 从这个物种。

项目成果

Stimulation of cAMP accumulation and lipolysis in hamster adipocytes with forskolin.

用毛喉素刺激仓鼠脂肪细胞中 cAMP 的积累和脂肪分解。

• DOI: 10.1152/ajpcell.1984.246.1.c63
• 发表时间: 1984
• 期刊: The American journal of physiology
• 作者: Schimmel,RJ

Inhibition of forskolin activated adenylate cyclase in hamster adipocyte membranes with prostaglandin E1 and clonidine.

用前列腺素 E1 和可乐定抑制仓鼠脂肪细胞膜中毛喉素激活的腺苷酸环化酶。

• 发表时间: 1982
• 期刊: Journal of cyclic nucleotide research
• 作者: McMahon,KK;Schimmel,RJ
• 通讯作者: Schimmel,RJ

Inhibition of cyclic AMP accumulation in hamster adipocytes with phosphatidic acid: differences and similarities with alpha adrenergic effects.

用磷脂酸抑制仓鼠脂肪细胞中环磷酸腺苷的积累：与α肾上腺素能作用的差异和相似之处。

• 发表时间: 1980
• 期刊: Journal of cyclic nucleotide research
• 作者: Schimmel,RJ;Honeyman,TW;McMahon,KK;Serio,R;Clark,RB
• 通讯作者: Clark,RB

Effects of 9-beta-D-arabinofuranosyl-guanine-5'-triphosphate on adenylate cyclase: comparison with GTP and GPP(NH)P.

9-β-D-阿拉伯呋喃糖基-鸟嘌呤-5-三磷酸对腺苷酸环化酶的影响：与 GTP 和 GPP(NH)P 的比较。

• 发表时间: 1983
• 期刊: Journal of cyclic nucleotide and protein phosphorylation research
• 作者: McMahon,KK;Hosey,MM;Schimmel,RJ
• 通讯作者: Schimmel,RJ

Alpha-adrenergic inhibition of cyclic AMP accumulation in hamster adipocytes. Similarity of receptor with alpha-2 adrenergic receptors.

α-肾上腺素能抑制仓鼠脂肪细胞中环 AMP 的积累。

• DOI: 10.1016/0304-4165(80)90331-1
• 发表时间: 1980
• 期刊: Biochimica et biophysica acta
• 作者: Schimmel,RJ;Serio,R;McMahon,KK
• 通讯作者: McMahon,KK