NON-PROTEIN AMINO ACID BIOCHEMISTRY AND TOXICITY

非蛋白质氨基酸生物化学和毒性

• 批准号: 3151051
• 负责人: GERALD A ROSENTHAL
• 金额: $ 7.54万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3151051
• 关键词: Leguminoseae; aminoacid analog; aminoacid biosynthesis; antimetabolites; arginine; colorimetry; cytotoxicity; electron microscopy; electron spin resonance spectroscopy; erythrocyte membrane; gel electrophoresis; membrane structure; nucleic acid metabolism; ornithine; plants; radiotracer; tissue /cell culture; urea cycle

Higher plants synthesize a diversified array of non-protein amino acids many of which bear marked structural analogy to the amino acids of mammalian metabolism. As a result, these secondary plant constituents are usually highly toxic and exhibit pronounced antimetabolic properties in animals. A particular group of naturally occurring non-protein amino acids, namely L-canavanine, L-canaline, and O-ureido-L-homoserine, are structurally related to the intermedates of the Krebs-Henseleit ornithine-urea cycle of animals. This group of non-protein amino acids will be utilzed as a model for understanding the biochemical basis for the toxicity and mode of action of these toxic amino acids. Emphasis will be placed on improving the utility of these compounds in fundamental biochemical studies.

较高的植物合成了多样化的非蛋白质氨基酸阵列许多 具有与哺乳动物代谢的氨基酸的结构类比。 结果，这些二级植物成分通常是剧毒的，并且 在动物中表现出明显的抗代谢特性。 特定的一组天然存在的非蛋白质氨基酸，即 l-甲烷，l-甲拉链和o- ureido-l-homoserine在结构上相关 动物的克雷布斯·亨格利特鸟氨酸周期的中间物。 这 一组非蛋白质氨基酸将被用作理解的模型 这些有毒氨基的毒性和作用方式的生化基础 酸。 重点将放在改善这些化合物的实用性上 基本生化研究。

项目成果

A mechanism of L-canaline toxicity.

L-canaline 毒性的机制。

• DOI: 10.1111/j.1432-1033.1981.tb05149.x
• 发表时间: 1981
• 期刊: European journal of biochemistry
• 作者: Rosenthal,GA

A radiometric assay for determining the incorporation of L-canavanine or L-arginine into protein.

用于确定 L-刀豆氨酸或 L-精氨酸掺入蛋白质的放射测定法。

• DOI: 10.1016/0003-2697(85)90292-1
• 发表时间: 1985
• 期刊: Analytical biochemistry
• 影响因子: 2.9
• 作者: Rosenthal,GA;Thomas,DA
• 通讯作者: Thomas,DA

Radiochemical synthesis of L-[guanidinooxy-14C]canavanine.

L-[胍基氧基-14C]刀豆氨酸的放射化学合成。

• DOI: 10.1016/0003-2697(83)90083-0
• 发表时间: 1983
• 期刊: Analytical biochemistry
• 影响因子: 2.9
• 作者: Rosenthal,GA;Downum,KR;Mattler,JE
• 通讯作者: Mattler,JE

In vitro incorporation of L-canavanine into vitellogenin of the fat body of the migratory locust Locusta migratoria migratorioides.

体外将 L-刀豆氨酸掺入飞蝗 Locusta migratoria migratorioides 脂肪体的卵黄蛋白原中。

• DOI: 10.1073/pnas.78.9.5480
• 发表时间: 1981
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Pines,M;Rosenthal,GA;Applebaum,SW
• 通讯作者: Applebaum,SW