MECHANISMS OF PROGRAMMED CELL DEATH

程序性细胞死亡的机制

• 批准号: 2699726
• 负责人: ZAHRA ZAKERI
• 金额: $ 8.29万
• 依托单位: QUEENS COLLEGE
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2699726
• 关键词: Drosophilidae; Manduca; RNA biosynthesis; animal genetic material tag; apoptosis; complementary DNA; developmental genetics; gene expression; gene mutation; genetic library; laboratory mouse; limbs; metamorphosis; northern blottings; nucleic acid hybridization; nucleic acid sequence; organ culture; polymerase chain reaction; protein biosynthesis; retinoate; sulfated glycoprotein 2; vertebrate embryology

During the tenure of a K04, I intend to pursue the following specific questions: 1. Analyze the molecular biology of normal, mutant, and retinoic-acid-induced cell death in the interdigital region of the developing mouse by examination of light- and electron microscopic morphology, DNA fragmentation; RNA and protein synthesis, and expression of genes associated with cell death such as transglutaminase, TRPM-2 and Bcl-2). 2) Establish an in vitro organ culture system as a model for studying limb development and cell death. II. Isolate genes related to programmed cell death: A. Establishment of stage-specific cDNA libraries for isolation of developmental stage specific and cell death related genes. B. Isolate and characterize common cDNAs activated during involution of Manduca labial gland and muscle.

在K04任职期间，我打算追求以下具体目标 问题： 1. 分析正常、突变和 视黄酸诱导的指间区域细胞死亡 通过光学和电子显微镜检查发育小鼠 形态学、DNA 片段化； RNA和蛋白质的合成和表达 与细胞死亡相关的基因，例如转谷氨酰胺酶、TRPM-2 和 Bcl-2）。 2）建立体外器官培养系统作为模型 研究肢体发育和细胞死亡。 二.分离相关基因 程序性细胞死亡：A.阶段特异性cDNA文库的建立 用于分离发育阶段特异性和细胞死亡相关的 基因。 B. 分离并表征在过程中激活的常见 cDNA 曼杜卡唇腺和肌肉的复旧。

项目成果

Rescue of the limb deformity in hammertoe mutant mice by retinoic acid-induced cell death.

通过视黄酸诱导的细胞死亡来拯救锤状趾突变小鼠的肢体畸形。

• 发表时间: 1997-04
• 作者: Ahuja, H S;James, W;Zakeri, Z
• 通讯作者: Zakeri, Z

Association of cyclin-dependent kinase 5 and its activator p35 with apoptotic cell death.

细胞周期蛋白依赖性激酶 5 及其激活剂 p35 与细胞凋亡的关联。

• 发表时间: 1997
• 作者: Ahuja, H S;Zhu, Y;Zakeri, Z
• 通讯作者: Zakeri, Z

Cell death in the heart.

心脏细胞死亡。

• DOI: 10.1016/s0733-8651(05)70191-0
• 发表时间: 2001-02-01
• 期刊: Cardiology clinics
• 影响因子: 2.4
• 作者: R. Lockshin;C. Facey;Z. Zakeri
• 通讯作者: Z. Zakeri

Coenzyme Q10 can in some circumstances block apoptosis, and this effect is mediated through mitochondria.

辅酶 Q10 在某些情况下可以阻止细胞凋亡，这种作用是通过线粒体介导的。

• 发表时间: 1999
• 影响因子: 5.2
• 作者: Kagan, T;Davis, C;Lin, L;Zakeri, Z
• 通讯作者: Zakeri, Z

Expression of clusterin in cell differentiation and cell death.

凝聚素在细胞分化和细胞死亡中的表达。

• 发表时间: 1994-11
• 作者: Ahuja, H S;Tenniswood, M;Lockshin, R;Zakeri, Z F
• 通讯作者: Zakeri, Z F