STAGE SPECIFIC TRANSCRIPTS IN HUMAN MALARIA PARASITES

人类疟疾寄生虫阶段特异性转录

• 批准号: 3145150
• 负责人: ALEXANDRA M LEVITT
• 金额: $ 15.24万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-03-01 至 1994-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3145150
• 关键词: Plasmodium; Plasmodium berghei; Plasmodium falciparum; RNA biosynthesis; RNA splicing; Saimiri; centrifugation; complementary DNA; developmental genetics; gel electrophoresis; gene expression; genetic regulation; genetic techniques; genetic transcription; life cycle; malaria; method development; microorganism genetics; molecular cloning; nucleic acid hybridization; nucleic acid sequence; polymerase chain reaction

Our long-range objective is to study the regulation of gene expression in human malarial parasites at the level of RNA processing and transcriptional control. As a first step, we are developing methods for the amplification and isolation of specific malarial cDNA ends, using the polymerase chain reaction technique. The fact that plasmodial ribosomes are stage-specific suggests that transcripts from the sexual and asexual stages of the parasite's lifecycle may differ in significant ways. Our preliminary data suggests that the 3' ends of transcripts coding for the circumsporozoite (CS) gene of the murine malaria P. berghei may be processed differently in sporozoites and blood stage parasites. Our intention is to extend this analysis to tbe CS genes of the human malarias P. falciparum and P. vivax. To determine whether alternative processing is a peculiarity of tbe CS gene or a general stage-specific mechanism, we will also examine the transcripts of three or more genes of P. falciparum and P. vivax that code for household enzymes or structural proteins expressed in all stages. Malarial cDNA's generated from sporozoite and blood stage RNA samples will be analyzed for 5' leader sequences, internal splicing patterns, polyadenylation sites, trans-splicing and RNA editing.

我们的远程目标是研究基因表达的调节 人类疟疾寄生虫在RNA加工和转录水平上 控制。 作为第一步，我们正在开发扩增的方法 并使用聚合酶链分离特定的疟疾cDNA末端 反应技术。 质体核糖体是特定于阶段的事实表明 寄生虫生命周期的性和无性阶段的成绩单 可能以重大的方式有所不同。我们的初步数据表明3' 编码鼠的孔子（CS）基因的转录本的末端 疟疾P. berghei在孢子和血液中的处理方式可能有所不同 阶段寄生虫。 我们的目的是将此分析扩展到CS基因 人类疟疾P.恶性疟原虫和Vivax。 确定替代处理是否是TBE CS基因的特殊性 或特定阶段的机制，我们还将检查成绩单 falciparum和P. vivax的三个或更多基因的代码 在各个阶段表达的家庭酶或结构蛋白。 疟疾 由Sporozoite和血液阶段RNA样品产生的cDNA将是 分析了5'领导者序列，内部剪接模式， 聚腺苷酸位点，变速器和RNA编辑。