CELL RECEPTOR FOR VISNA--MACROPHAGE-TROPIC LENTIVIRUS

VISNA细胞受体--巨噬细胞热带慢病毒

• 批准号: 3147396
• 负责人: JANICE E CLEMENTS
• 金额: $ 18.54万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-02-01 至 1997-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3147396
• 关键词: Retroviridae; Retroviridae disease; epitope mapping; immunoaffinity chromatography; laboratory rabbit; macrophage; molecular cloning; monocyte; virus infection mechanism; virus receptors

Lentiviruses are non-oncogenic retroviruses that cause persistent infection and chronic disease after prolonged inoculation periods. The viruses include the human immunodeficiency viruses (HIV) and simian immunodeficiency viruses (SIV) as well as lentiviruses of Feline, Bovine and Equine species and the ungulate lentiviruses. Lentiviruses cause two types of disease; immunodeficiency disease as a result of loss of helper T lymphocytes and organ specific disease in brain, lung and intestine associated with replication in macrophages. HIV (in adults) and SIV cause both types of disease, pediatric AIDS is characterized by more organ specific disease, and the ungulate lentiviruses (visna virus and caprine arthritis encephalitis virus, CAEV) cause only the organ specific disease. The target cell for the immunodeficiency viruses is CD4 lymphocytes as well as macrophages. However, the ungulate lentivirus, visna virus and CAEV, target the monocyte/macrophages. The receptor for the immunodeficiency viruses is the CD4 molecule on lymphocytes, but the receptor for the macrophage tropic lentiviruses has not been identified. Visna virus binds to a molecule on virus susceptible cells. Antibody to this 5OkD surface protein specifically blocks the binding of visna virus to cells. These data provide the basis for isolation of molecular clones of the visna virus receptor. Molecular cloning of the receptor will allow the characterization of the receptor molecule and identification of it in target organs. Characterization of the receptor for the ungulate lentiviruses will provide a molecular tool to understand the basis for the organ specific disease caused by these viruses and may provide leads to understanding how the immunodeficiency virus cause organ specific disease.

慢病毒是引起持续感染的非癌逆转录病毒 和慢性接种期后的慢性疾病。 病毒 包括人类免疫缺陷病毒（HIV）和邻苯二豆 免疫缺陷病毒（SIV）以及猫，牛的慢病毒 和马种和烟病毒。 慢病毒引起两个 疾病类型；免疫缺陷疾病由于助手损失而导致 大脑，肺和肠道中的淋巴细胞和器官特异性疾病 与巨噬细胞中的复制有关。 艾滋病毒（成人）和SIV原因 两种类型的疾病，小儿艾滋病的特征是更多的器官 特异性疾病以及解剖疾病病毒（粘膜病毒和caprine） 关节炎病毒，CAEV）仅引起器官特异性疾病。 免疫缺陷病毒的靶细胞也是CD4淋巴细胞 作为巨噬细胞。 然而，慢跑病毒，维斯纳病毒和CAEV， 靶向单核细胞/巨噬细胞。 免疫缺陷的受体 病毒是淋巴细胞上的CD4分子，但是 巨噬细胞热带慢病毒尚未鉴定。 维斯纳病毒与病毒易感细胞上的分子结合。 抗体 该5OKD表面蛋白特异性地阻断了Visna病毒与 细胞。 这些数据为隔离分子克隆的基础 维斯纳病毒受体。 受体的分子克隆将允许 受体分子的表征及其在中的鉴定 目标器官。 受体的表征 慢病毒将提供一个分子工具，以了解 这些病毒引起的器官特异性疾病，可能会导致 了解免疫缺陷病毒如何引起器官特定疾病。