IN VITRO STUDIES ON CRYPTOSPORIDIUM, AN OI OF AIDS

艾滋病病原体隐孢子虫的体外研究

• 批准号: 3146745
• 负责人: Steve J Upton
• 金额: $ 13.73万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3146745
• 关键词: AIDS; Cryptosporidium; cell type; cryptosporidiosis; goats; growth media; microorganism growth; opportunistic infections; oxygen; tissue /cell culture

Cryptosporidium parvum (Apicomplexa) has emerged as an important opportunistic pathogen of patients suffering from the Acquired Immunodeficiency Syndrome (AIDS). Gastrointestinal involvement may become so severe in victims of AIDS that death due to diarrheal dehydration and electrolyte imbalance may occur. Our laboratory is currently examining the basic biology of surface and endogenous proteins and glycoproteins of sporozoites of C. parvum, and have developed methods of studying the parasite in vitro. Based on our preliminary observations, we believe methods can be developed whereby pharmaceutical screening and mass cultivation of C. parvum can be achieved. We propose to study the effects of atmospheric 02, host cell type, and nutritional additives in order to develop optimal conditions by which C. parvum can be studied in cultured cells.

隐孢子虫（Apicomplexa）已成为重要的 遭受收购的患者的机会性病原体 免疫缺陷综合征（AIDS）。胃肠道参与可能会变成 艾滋病受害者如此严重，以至于由于腹泻脱水而导致的死亡 可能发生电解质不平衡。 我们的实验室目前正在研究 表面和内源性蛋白质和糖蛋白的基本生物学 C. parvum的子孢子，并开发了研究的方法 体外寄生虫。 根据我们的初步观察，我们相信 可以开发出药物筛查和质量的方法 可以实现C. parvum的种植。 我们建议研究效果 大气02，宿主细胞类型和营养添加剂的目的 发展最佳条件 细胞。