Mapping resistance to Campylobacter in the chicken

绘制鸡对弯曲杆菌的耐药性

• 批准号: BB/J006815/1
• 负责人: Mark Stevens
• 金额: $ 59.89万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2012
• 资助国家: 英国
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FJ006815%2F1
• 关键词: Mapping; resistance; Campylobacter; chicken

Food security is an increasing priority for the UK Government and food safety is a key component of this. Campylobacter is the most common cause of food poisoning in the UK and is responsible for an estimated 321,000 estimated cases in England and Wales in 2008, with over 15,000 hospitalisations and 76 deaths. Campylobacter accounts for a third of the cost of food-borne illness in England and Wales, estimated at £583 million in 2008. It is found mainly in poultry and control of the organism in this reservoir is expected to lower the inclidence of human infection."Campylobacter is a highly complex organism and we will only be able to understand it more fully and to overcome the threat it poses by deploying world-class research across microbiology, immunology and molecular biology... Addressing the Campylobacter challenge involves two of the three BBSRC strategic priorities of food security and basic bioscience underpinning health, in addition to exploiting systems approaches and new research tools." Professor Douglas Kell, BBSRC CEO.The UK Research and Innovation Strategy for Campylobacter, 2010-2015, is joint funded by the FSA, BBSRC, DEFRA, DARDNI and the Scottish Government. The overarching aim for the funders is to reduce the incidence of Campylobacter infections in humans, through reduction of the level of the bacterium in its farm animal hosts and the potential for cross-contamination in the food chain. Two of the main research priorities are understanding colonisation in the chicken and the chicken's immune response, and development of greater resistance to Campylobacter colonisation in the chicken. However, research in either of these areas was not included in a recent joint call for research into control of Campylobacter.The availability of chickens with significant innate resistance to Campylobacter colonisation would represent a safe, cost-effective solution. We have shown that genetic variation influences resistance to Campylobacter colonisation in chickens, with one line able to reduce levels of the bacterium in the gut by up 10,000-fold relative to another line. Complex genetic analysis in these inbred chicken lines has indicated that some of the regions, or loci, associated with resistance overlie known bacterial resistance genes. In this proposal we will investigate these loci further to locate the beneficial genes or mutations.We will carry out further work to map resistance genes, coupled with using the latest methods that allow selection of genes throughout the whole genome. Using tools such as new generations of high density panels to detect single nucleotide polymorphisms (changes in a single base of DNA between two different individuals at the same site) (~750k can be analysed at once), we can identify animals with candidate resistance genes that can be used to select future generations of chickens with robust resistance and greatly reduced levels of Campylobacter colonisation. As recommended by the Strategy, we will carry out this genetic research in collaboration with industry (Aviagen, a UK-based company that produces the pedigree chickens from which are generated over 50% of the world's 50 billion chickens every year), since gene association studies must be implemented in poultry breeding programmes. It is likely that resistance to Campylobacter depends on multiple genes and the selection of resistant lines will depend on the identification of specific traits and candidate animals. Though potential also exists to control Campylobacter on farm via improvements to hygiene and biosecurity, dietary modification, novel antimicrobial agents and vaccination, we believe that the proposed project would allow a targeted and rapid approach to breeding lines of poultry more resistant to Campylobacter colonisation.

粮食安全是英国政府，粮食安全是英国的关键组成部分。 2008年的成本Inness Ingland Angland and Wales llion。它主要是在家禽中发现的，并且该储层中对生物体的控制被曝光以降低人类感染的含量。和分子生物学...应对弯曲杆菌的挑战涉及TOOD Cecurity和Bioscieng Bioscieng Bioscieng Bioscieng Health STEMS方法和新的研究工具对于人类的ct虫感染而言，这是对农场动物宿主的水平以及食物链中交叉污染的潜力，并发展出对鸡肉弯曲杆菌的抗性的可能性，在这两个地区都没有进行研究。为了使肠道中的某些区域或基因座降低10,000倍。使用该方法绘制抗性基因的进一步绘制基因。选择具有抵抗力和巨大水平的鸡的未来后代，与工业公司合作（Aviagen）（英国的公司，生产来自WICH的谱系鸡的aviagen）是全球50只比尔（Aviage）抗性线的选择将取决于对特定性状和动物的识别，尽管潜在的Alsol控制弯曲杆菌是通过非斜率在农场上通过卫生和生物安全性，新颖的OBIAL剂和疫苗接种的方法，但我们相信该项目我会允许一个目标和快速的善良和良好的方法家禽的繁殖更多TT弯曲杆菌定殖。

项目成果

The genomic architecture of resistance to Campylobacter jejuni intestinal colonisation in chickens.

• DOI: 10.1186/s12864-016-2612-7
• 发表时间: 2016-04-18
• 期刊: BMC genomics
• 影响因子: 4.4
• 作者: Psifidi A;Fife M;Howell J;Matika O;van Diemen PM;Kuo R;Smith J;Hocking PM;Salmon N;Jones MA;Hume DA;Banos G;Stevens MP;Kaiser P
• 通讯作者: Kaiser P

Colonization of a commercial broiler line by Campylobacter is under limited genetic control and does not significantly impair performance or intestinal health.

• DOI: 10.3382/ps/pey295
• 发表时间: 2018-12-01
• 期刊: Poultry science
• 影响因子: 4.4
• 作者: Bailey RA;Kranis A;Psifidi A;Watson KA;Rothwell L;Hocking PM;Kaiser P;Stevens MP;Avendano S
• 通讯作者: Avendano S

Quantitative trait loci and transcriptome signatures associated with avian heritable resistance to Campylobacter.

• DOI: 10.1038/s41598-020-79005-7
• 发表时间: 2021-01-12
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Psifidi A;Kranis A;Rothwell LM;Bremner A;Russell K;Robledo D;Bush SJ;Fife M;Hocking PM;Banos G;Hume DA;Kaufman J;Bailey RA;Avendano S;Watson KA;Kaiser P;Stevens MP
• 通讯作者: Stevens MP

Genetic control of Campylobacter colonisation in broiler chickens: genomic and transcriptomic characterisation

肉鸡中弯曲杆菌定植的遗传控制：基因组和转录组特征

• DOI: 10.1101/2020.04.14.040832
• 发表时间: 2020
• 作者: Psifidi A

Combination of novel and public RNA-seq datasets to generate an mRNA expression atlas for the domestic chicken.

• DOI: 10.1186/s12864-018-4972-7
• 发表时间: 2018-08-07
• 期刊: BMC genomics
• 影响因子: 4.4
• 作者: Bush SJ;Freem L;MacCallum AJ;O'Dell J;Wu C;Afrasiabi C;Psifidi A;Stevens MP;Smith J;Summers KM;Hume DA
• 通讯作者: Hume DA