GENETIC AND MOLECULAR BASIS OF BACTERIAL INVASION

细菌入侵的遗传和分子基础

• 批准号: 3139914
• 负责人: STANLEY FALKOW
• 金额: $ 27.71万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3139914
• 关键词: Enterobacteriaceae; Enterobacteriaceae disease; Escherichia coli; Salmonella; Salmonella infections; active transport; affinity chromatography; autoradiography; bacterial antigens; bacterial cytopathogenic effect; bacterial genetics; bacterial proteins; basolateral membrane; diarrhea; enteric bacteria; gastrointestinal epithelium; gastrointestinal infection; gel electrophoresis; gene expression; genetic recombination; genetic strain; host organism interaction; membrane proteins; microorganism culture; molecular cloning; nucleic acid sequence; plasmids; receptor; receptor mediated endocytosis; tissue /cell culture; virulence

The object of the research proposed here is to gain a better understanding of both the genetic and molecular basis of the interaction of enteric microorganisms with epithelial cells. We have focused on three aspects of this interaction: entry of the bacteria (Yersinia and Salmonella) into epithelial cells; replication of the bacteria within the epithelial cell; and transcytosis of the bacteria through the epithelial cell. We will continue to characterize the invasion determinants, inv and ail, of Yersinia both genetically and biochemically. In vitro modified inv and ail genes will be returned to Yersinia to better assess the role these determinants play in the virulence of this organism. In addition, we will extend our studies with Salmonella to identify and characterize the genes (and their products) involved in invasion and intracellular replication. Our preliminary work in these areas has been most encouraging and indicates these aspects of microbial pathogenesis are amenable to experimental investigation.

这里提出的研究目的是为了获得更好的 了解遗传和分子基础 肠道微生物与上皮细胞的相互作用。 我们 重点关注了这种互动的三个方面： 细菌（耶尔森氏菌和沙门氏菌）进入上皮细胞；复制 上皮细胞内的细菌；和转胞吞作用 细菌通过上皮细胞。 我们将继续 描述耶尔森氏菌的入侵决定因素 inv 和 ail 无论是遗传上还是生化上。 体外修饰的 inv 和 ail 基因将被送回耶尔森氏菌，以更好地评估这些基因的作用 决定因素决定了该生物体的毒力。 此外， 我们将扩大对沙门氏菌的研究，以识别和 表征参与入侵的基因（及其产物） 和细胞内复制。 我们在这些方面的前期工作 是最令人鼓舞的，并表明了这些方面 微生物发病机制适合实验 调查。