Dissecting the diverse development programmes in different tissues during the development of a nitrogen fixing nodule

剖析固氮根发育过程中不同组织的不同发育程序

• 批准号: BB/J001872/1
• 负责人: Giles Oldroyd
• 金额: $ 67.54万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2012
• 资助国家: 英国
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FJ001872%2F1
• 关键词: Dissecting; diverse; development; programmes; different

Plants must acquire the elemental nutrient nitrogen from their surrounding environment and its availability is often a major limitation to plant growth. Legumes (peas and beans) have a unique strategy to deal with nitrogen limitation. They enter symbiotic interactions with soil bacteria that are able to fix an atmospheric form of nitrogen that plants cannot take up, and convert it into the biologically active form ammonia. This interaction involves the entry of the bacteria into the roots of the legume plant together with development of a 'nodule', a root outgrowth that houses the bacteria. Co-ordination of bacterial entry with cell division that leads to the formation of the nodule is essential to ensure a bacterially infected and nitrogen fixing nodule. We have uncovered some of the key genes that control nodulation responses in the outermost layer of the root, the epidermis, where bacteria enter the plant. We have also identified genes that regulate responses several layers beneath the epidermis, in the cortical cell layer where the nodule structure develops. Intriguingly there appears to be genetic overlap between these two response systems, suggesting that the function of a gene is defined by the cell type where it is active. In this proposal we will assess the mechanisms of nodulation signaling in the root epidermis and the root cortex and in particular will focus on the role of transcription factors that regulate gene expression. We will combine genetic approaches with state of the art genomic approaches to develop a systems level understanding of this process. In particular we will attempt to define the roles of the transcription factors in the different tissues and the mechanisms by which these transcription factors are able to coordinate diverse developmental programmes in different tissues. This will enable us to define the mechanisms that allow coordinated plant development leading to the formation of the nitrogen fixing nodule.

植物必须从周围环境中获取元素营养氮，其可用性通常是植物生长的主要限制。豆类（豌豆和蚕豆）有独特的策略来应对氮限制。它们与土壤细菌发生共生相互作用，土壤细菌能够固定植物无法吸收的大气形式的氮，并将其转化为具有生物活性的氨。这种相互作用涉及细菌进入豆科植物的根部，并形成“根瘤”，即容纳细菌的根部生长物。细菌进入与导致结节形成的细胞分裂的协调对于确保细菌感染和固氮结节至关重要。我们发现了一些控制根部最外层（即表皮，细菌进入植物的地方）结瘤反应的关键基因。我们还发现了调节表皮下几层反应的基因，即结节结构发育的皮质细胞层。有趣的是，这两个反应系统之间似乎存在遗传重叠，这表明基因的功能是由其活跃的细胞类型决定的。在本提案中，我们将评估根表皮和根皮层中结瘤信号传导的机制，特别是关注调节基因表达的转录因子的作用。我们将把遗传方法与最先进的基因组方法结合起来，以开发对这一过程的系统级理解。特别是，我们将尝试定义转录因子在不同组织中的作用以及这些转录因子能够协调不同组织中不同发育程序的机制。这将使我们能够确定植物协调发育导致固氮根形成的机制。

项目成果

The identification of novel loci required for appropriate nodule development in Medicago truncatula.

• DOI: 10.1186/1471-2229-13-157
• 发表时间: 2013-10-11
• 期刊: BMC plant biology
• 影响因子: 5.3
• 作者: Domonkos A;Horvath B;Marsh JF;Halasz G;Ayaydin F;Oldroyd GE;Kalo P
• 通讯作者: Kalo P