MORPHOLOGICAL DYNAMICS OF LIVING NEURONS IN SLICES

切片中活神经元的形态动力学

• 批准号: 2609542
• 负责人: Steve M Potter
• 金额: $ 3.15万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-11-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2609542
• 关键词: cell growth regulation; cell transplantation; developmental neurobiology; hippocampus; laboratory rat; mammalian embryology; nervous system transplantation; neurogenesis; neurons; tissue /cell culture

This study explores the potentials of a new organotypic culture system as a model for in vivo neural transplants, and as a tool for studying the morphological correlates of synaptic plasticity. I will study the development of embryonic rat hippocampal neurons transplanted to cultured hippocampal slices from neonatal rats. I will observe the process of transplant integration while it happens, using time-lapse 2-photon microscopy. I will observe the effects of slice age and transplant location on the neurite outgrowth of transplanted CA1 and CA3 pyramidal neurons. I will quantify dynamic morphological parameters of the transplanted cells, such as neurite growth and retraction rates, branch formation, and cell body migration, in comparison to those of the host cells. Depolarizing agents will be applied to slices during timelapse imaging to see how the overall level of spontaneous activity in the slice influences neural morphology. The findings will be useful to those interested in the bases of learning and memory, and the potential for hippocampal transplants to ameliorate the effects of neurodegenerative disorders, such as Alzheimer's disease.

这项研究探索了新的器官型培养系统的潜力 作为体内神经移植的模型，以及作为研究的工具 突触可塑性的形态相关性。我将研究 胚胎大鼠海马神经元移植培养的发育 来自新生大鼠的海马切片。 我会观察这个过程 使用延时 2 光子进行移植整合 显微镜。我将观察切片年龄和移植的影响 移植的 CA1 和 CA3 锥体神经突生长的位置 神经元。 我将量化动态形态参数 移植细胞，例如神经突生长和回缩率、分支 与宿主的形成和细胞体迁移相比 细胞。在延时摄影期间，去偏振剂将应用于切片 成像以了解切片中自发活动的总体水平如何 影响神经形态。 研究结果将对那些人有用 对学习和记忆的基础以及潜力感兴趣 海马移植可改善神经退行性疾病的影响 疾病，例如阿尔茨海默病。

项目成果

Precisely timed spatiotemporal patterns of neural activity in dissociated cortical cultures.

分离皮质培养物中神经活动的精确定时时空模式。

• 发表时间: 2007-08-10
• 影响因子: 3.3
• 作者: Rolston, J D;Wagenaar, D A;Potter, S M
• 通讯作者: Potter, S M

Neural transplant staining with DiI and vital imaging by 2-photon laser-scanning microscopy.

使用 DiI 进行神经移植染色，并通过 2 光子激光扫描显微镜进行生命成像。

• 发表时间: 1996
• 作者: Potter, S M;Pine, J;Fraser, S E
• 通讯作者: Fraser, S E

Structure and emergence of specific olfactory glomeruli in the mouse.

小鼠特定嗅觉肾小球的结构和出现。

• 发表时间: 2001-12-15
• 作者: Potter, S M;Zheng, C;Koos, D S;Feinstein, P;Fraser, S E;Mombaerts, P
• 通讯作者: Mombaerts, P