ADENYLATE CYCLASE OF BORDETELLA PERTUSSIS

百日咳博德特氏菌腺苷酸环化酶

• 批准号: 3134373
• 负责人: Richard Lee Friedman
• 金额: $ 15.33万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 1990-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3134373
• 关键词: Bordetella pertussis; adenylate cyclase; alveolar macrophages; bacteriophage lambda; chemical structure function; gel electrophoresis; human tissue; hybridomas; immunochemistry; microorganism genetics; molecular cloning; monoclonal antibody; monocyte; organ culture; pertussis; phagocytes; respiratory epithelium; structural genes; virulence

Pertussis (Whooping cough) is a prolonged, often devastating infection of the upper respiratory tract caused by Bordetella pertussis. B. pertussis produces a vast array of potential virulence factors but which are important in causing disease are not completely known. The goal of this research is to study one virulence factor of B. pertussis, the adenylate cyclase in order to obtain reagents and background information which are necessary for further studies on the possible role of this toxin in the disease. Specifically the proposed studies involve: (1) production and purification of the adenylate cyclase for subsequent characterization studies. (2) to characterize the purified adenylate cyclase chemically and to determine possbile subunit structure and function. (3) to characterize the purified adenylate cyclase biologically including studies on the effect of the cyclase on phagocytic/metabolic activities of human and rabbit phagocytic cells, interaction with respiratory epithelium and production of monoclonal and affinity purified polyclonal antibody for immunological and structure-function studies. (4) and attempt to understand the genetics of the cyclase by cloning its structural genes.

百日咳（百日咳）是一种长期的、往往具有毁灭性的疾病 博德特氏菌引起的上呼吸道感染 百日咳。 百日咳博德特氏菌产生巨大的潜力 毒力因子，但对引起疾病很重要的是 不完全知道。 这项研究的目的是研究一个 百日咳博德特氏菌的毒力因子，腺苷酸环化酶，以便 获取必要的试剂和背景信息 进一步研究这种毒素在疾病中的可能作用。 具体而言，拟议的研究涉及： (1)腺苷酸环化酶的制备和纯化 随后的表征研究。 (2) 对纯化的腺苷酸环化酶进行化学表征 并确定可能的亚基结构和功能。 (3) 对纯化的腺苷酸环化酶进行生物学表征 包括环化酶影响的研究 人和兔的吞噬/代谢活动 吞噬细胞、与呼吸道上皮细胞的相互作用以及 单克隆和亲和纯化多克隆的生产 用于免疫学和结构功能研究的抗体。 (4) 并尝试通过以下方式了解环化酶的遗传学 克隆其结构基因。