REGULATION OF POLYSACCHARIDE SYNTHESIS

多糖合成的调控

• 批准号: 3134412
• 负责人: JACK PREISS
• 金额: $ 21.73万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-05-01 至 1986-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3134412
• 关键词: Enterobacteriaceae; Escherichia coli; Escherichia coli k12; Rhodospirillum; affinity chromatography; allosteric site; bacterial genetics; carbohydrate biosynthesis; enzyme structure; gel electrophoresis; gel filtration chromatography; genetic manipulation; genetic mapping; genetic recombination; genetic strain; genetic transcription; genetic translation; glycogen; glycogen synthase; glycolipids; glycosyltransferase; immunochemistry; microorganism genetics; molecular cloning; mutant; nucleic acid sequence; other phosphotransferase; photosynthetic bacteria; radiotracer; structural genes

It is proposed to continue studies on the bacterial glycogen biosynthetic enzymes. Emphasis will be on the regulatory and catalytic properties of the ADPglucose pyrophosphorylase from E. coli B and from various photosynthetic bacteria. Chemical modification studies will be used to characterize and define the allosteric effector and catalytic sites of the enzyme. Comparative studies at the protein sequence level will be made in order to relate structure with function and specificity at these various ligand binding sites. Furthermore a study of kinetically altered ADPglucose pyrophosphorylases from E. coli glycogen mutants is initiated to determine the relationship of allosteric function with the primary structure of the ADPglucose pyrophosphorylase. The characterization of E. coli B glycogen synthase and branching enzyme with respect to reaction mechanism and the various amino acids involved in catalysis will be continued. Studies on the cloning of the glycogen biosynthetic structural genes of E. coli K12 will be continued so that future studies on DNA sequence analysis of the glg structural genes and on the genetic regulation of the biosynthesis of the glycogen biosynthetic enzymes can be initiated.

建议继续研究细菌糖原生物合成 酶。 重点将放在调节性和催化特性上 来自大肠杆菌B和各种光合作用的Adpglucose焦磷酸化酶 细菌。 化学修饰研究将用于表征和定义 酶的变构效应子和催化位点。 比较研究 将在蛋白质序列水平上进行，以将结构与 这些各种配体结合位点的功能和特异性。 此外 研究了大肠杆菌糖原的动力学改变的Adpglucose焦磷酸酶 启动突变体是为了确定变构功能与 Adpglucose焦磷酸化酶的主要结构。 表征 大肠杆菌B糖原合酶和分支酶相对于反应 将继续进行催化的机理和各种氨基酸。 关于大肠杆菌糖原生物合成结构基因的克隆的研究 K12将继续进行，以便将来对GLG的DNA序列分析进行研究 结构基因以及生物合成的遗传调节 可以启动糖原生物合成酶。