BIOLOGICAL CONROL OF MOSQUITOES WITH LAMBORNELLA CLARKI

用兰博内拉氏菌对蚊子进行生物防治

• 批准号: 3129780
• 负责人: JOHN R ANDERSON
• 金额: $ 8.25万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-08-01 至 1989-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3129780
• 关键词: Tetrahymena; arthropod borne communicable disease; genetic manipulation; host organism interaction; insect control; life cycle; microorganism culture; virulence

The broad objectives are to further illucidate the life cycle of Lambornella clarki, (Tetrahymenidae: Ciliphora) and to assess its potential as a manipulated biological control agent of the western treehole mosquito, Aedes sierrensis, and related mosquitoes. We also plan to establish L. clarki in known L. clarki-negative treeholes and to determine factors influencing infection rates and persistence in mosquito breeding sites. Specific laboratory objectives include determining environmental and physiological cues that stimulate encystation and excystation, determining how long cysts remain viable, and studying L. clarki and its pathogenesis for Ae. sierrensis and other mosquito species. The long-term objectives include development of an in vitro culture system for L. clarki, and ultimately development of techniques in laboratory mass-production, storage, and field inoculation of treeholes. Dispersal of the parasite by infected adults will be quantified under field conditions. Laboratory colonies will be used to study interrelationships between L. clarki and other pathogens. Laboratory mass production and field application of this mosquito pathogen could result in the successful use of a biological control agent with excellent potential for persistence in aquatic habitats of container-breeding mosquitoes. Container-type mosquito habitas are breeding sites for some of the most effective mosquito vectors of important human pathogens (e.g. Ae. aegypti and Yellow Fever and Dengue Fever, Ae. polynesiensis and Filariasis, Ae. albopictus and Dengue, and Ae. triseriatus and LaCrosse Virus). In California, Ae. sierrensis is a major biting pest, and it is a known vector of dog heartworm (Dirofilaria immitis) and the deer body worm (Setaria yehi), both of which occasionally infect humans.

广泛的目标是进一步伪造 Lambornella Clarki，（四苯胺科：Ciliphora），并评估其 作为西方树孔的操纵生物控制剂的潜力 蚊子，埃德斯·塞伦西斯和相关蚊子。 我们还计划 在已知的Clarki阴性树孔中建​​立L. clarki，并确定 影响蚊子繁殖的感染率和持久性的因素 站点。 特定的实验室目标包括确定环境 以及刺激百科全和脱身的生理提示， 确定囊肿保持可行的时间，并研究Clarki L.及其 AE的发病机理。 sierrensis和其他蚊子物种。 长期 目标包括开发用于克拉克乳杆菌的体外培养系统， 并最终开发实验室批量生产技术， 存储和树洞的现场接种。 分散寄生虫 受感染的成年人将在现场条件下进行量化。 实验室 殖民地将用于研究克拉基乳杆菌和 其他病原体。 实验室批量生产和现场应用 蚊子病原体可能会成功使用生物学 在水生栖息地中具有巨大持久性潜力的控制剂 集装箱繁殖的蚊子。 集装箱型蚊子栖息地为 重要的一些重要蚊子媒介的繁殖地点 人类病原体（例如Ae。Aegypti和黄热病和登革热，AE。 Polynesiensis和Filariasis，AE。白化病和登革热和AE。 Triseriatus和Lacrosse病毒）。 在加利福尼亚，AE。 Sierrensis是主要的 咬害虫，它是狗心脏的已知矢量（dirofilaria 侵入性）和鹿体蠕虫（Setaria Yehi），偶尔两者偶尔 感染人类。