MEASLES VIRUS: THE VIRION AND ITS REPLICATIVE STRATEGY

麻疹病毒：病毒粒子及其复制策略

• 批准号: 3130259
• 负责人: Stephen A. Udem
• 金额: $ 21.5万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3130259
• 关键词: HeLa cells; genetic transcription; genetic translation; genome; immunochemistry; measles; microorganism reproduction; molecular pathology; radiotracer; virulence; virus envelope; virus infection mechanism; virus morphology; virus replication

This research proposal seeks to rigorously characterize the molecular biology of the acute measles virus infectious cycle, and of its progeny - the completed, infectious measles virion. Establishing the details of measles virus replication during acute, productive infection is prerequisite to the long-range goals of my laboratory's research program - those being the identification of the mechanism(s) by which persistent measles infections are established and maintained, and the assessment of the role played by such infection in the pathogenesis of chronic human disease. On the basis of our previous studies, we have selected the HeLa tissue culture suspension cell as the laboratory 'model' for the examination of the replicative strategy by which progeny virus are generated during acute, productive infection. Using methods and probes already developed, the synthesis and structure of the virus-specified macromolecules will be analyzed, and their role(s) in the reproduction of the infectious virion established. Particular attention will be given to as yet undefined features of measles virus and of its acute infectious cycle, including: (1) the pathways of viral structural protein synthesis, processing, assembly, and release as infectious progeny; (2) the transcription of viral mRNAs and the definition of the translation product each encodes; (3) genome replication; and (4) assessment of the possibility that non-structural information is encoded by the measles genome. Furthermore, the clarification of several of the above features of measles virus reproduction would be best addressed using highly simplified, well-defined, and easily manipulable in vitro systems. We, therefore, plan to pursue the development of in vitro transcription (and perhaps in vitro replication) system(s) with which to examine these virus-specified synthetic processes when removed from host-cell influences.

该研究建议试图严格地表征分子 急性麻疹的生物学病毒感染周期及其后代的生物学 - 完整的，传染性的麻疹病毒体。 建立细节 麻疹病毒在急性期间，生产性感染是 我实验室研究计划的远程目标的先决条件 - 那些是对持久性机制的识别 麻疹感染是建立和维护的，并评估 这种感染在慢性人的发病机理中所起的作用 疾病。 根据我们以前的研究，我们选择了HeLa组织 培养悬架细胞作为实验室“模型” 急性期间产生后代病毒的复制策略， 生产感染。 使用已经开发的方法和探针， 病毒指定的大分子的合成和结构将是 进行了分析，及其在传染病的繁殖中的作用 已确立的。 特别关注尚未定义 麻疹病毒及其急性感染周期的特征，包括： （1）病毒结构蛋白质合成，加工， 组装，并释放为传染性后代； （2）病毒的转录 mRNA和翻译产品的定义每个编码； （3） 基因组复制； （4）评估的可能性 非结构信息由麻疹基因组编码。 此外，阐明了上述麻疹的几个特征 最好使用高度简化的病毒繁殖来解决 定义明确且易于操纵的体外系统。 因此，我们计划 追求体外转录的发展（也许是在体外 复制）系统可以检查这些病毒指定的 当从宿主细胞影响中删除合成过程。